Serial volumetric analysis was performed using MATLAB (MathWorks, Natick, Massachusetts) and PowerPoint (Microsoft Corp, Redmond, Washington) software. Summed voxel projection images were stacked in PowerPoint to overlap identical areas on each of the scans using landmarks such as retinal vessels, optic nerve, and fovea. Because of small rotation of the handheld probe between imaging sessions, alignment of the rectangles required rotating and laterally shifting each rectangle. A 4.5 × 6.6-mm rectangular area was common to all scans and was traced over the stack in PowerPoint. For each scan, the boundaries of the rectangle were determined on the summed voxel projections, and the B-scans in the common area for each session were entered into MATLAB to determine the macular volume of each scan through summation of the corresponding segmented areas of retinal thickness. Macular volume was 8.49 mm3 immediately prior to surgery and 7.86 mm3 after posterior hyaloid elevation. Following air-fluid exchange with optic pit aspiration, the volume decreased to 7.25 mm3. At 1 and 9 months postoperatively, the macular volume was 7.00 and 6.15 mm3, respectively.