Amniotic membrane can act as a biological bandage contact lens to the ocular surface, with therapeutic anti-inflammatory properties. The analgesic benefits of this intervention are believed to be due to mechanical protection. Amniotic membrane is also used in the treatment of neurotrophic corneal disease. Substance P is a neurotransmitter released by C-terminal nerve endings. It mediates acute inflammation and is known to play a role in modulating pain sensation. Substance P is also known to promote proliferation of various cell types, including epithelial and nerve progenitor cells.1,2 In this in vitro pilot study, we investigated the relationship between amniotic membrane and substance P.