Gentian violet has long been thought to be nontoxic. However, using a standard rabbit eye irritation test, Ballantyne et al2 found that GV produced blepharitis, edema, and necrosis of the conjunctivae and nictitating membrane, keratitis, and elevation of intraocular pressure. Two subsequent studies3,4 discovered an increased incidence of corneal edema following the injection of GV solutions into the anterior chamber of the human eye during cataract surgery. Chang et al5 later discovered cell damage to rabbit corneal endothelium after exposure to GV. Furthermore, a dose-response relationship was exhibited in the study by Chang et al.5 Most recently, using a vital dye assay on a human donor cornea, Ide et al1 demonstrated that marking the DSAEK donor stromal surface with a GV marking pen damaged the corneal endothelium.