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Longitudinal Study of Retinal Nerve Fiber Layer Thickness and Ganglion Cell Complex in Traumatic Optic Neuropathy FREE

Akiyasu Kanamori, MD, PhD; Makoto Nakamura, MD, PhD; Yuko Yamada, MD, PhD; Akira Negi, MD, PhD
[+] Author Affiliations

Author Affiliations: Division of Ophthalmology, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.


Arch Ophthalmol. 2012;130(8):1067-1069. doi:10.1001/archophthalmol.2012.470.
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Optical coherence tomography (OCT) has been developed to objectively evaluate structural damage in vivo. The tool can be used to evaluate damage to the circumpapillary retinal nerve fiber layer (cpRNFL). Measurements of the cpRNFL play an important role in the diagnosis and management of patients with glaucoma. The recent introduction of spectral-domain OCT has enhanced the scan resolution and provides more reproducibility for image acquisition compared with time-domain OCT, a previous version of OCT. Furthermore, the RTVue (Optovue Inc), one of the spectral-domain OCT instruments, allows us to evaluate inner retinal layer thickness. Reference to the ganglion cell complex (GCC) includes the retinal nerve fiber layer, retinal ganglion cell layer, and inner plexiform layer. Some studies have demonstrated the usefulness of GCC as well as cpRNFL thickness as measured by RTVue in treating glaucomatous eyes.

Traumatic optic neuropathy (TON) causes acute axonal loss with severe vision loss. A few studies have demonstrated axonal loss through the use of time-domain OCT or scanning laser polarimetry after TON.13 Recently, Cunha et al4 compared cpRNFL loss and macular thinning in 3 patients with TON. However, to our knowledge, no study has performed a direct comparison between cpRNFL thickness and GCC after TON. We herein statistically evaluated longitudinal changes in cpRNFL thickness and the GCC in 4 patients with unilateral TON.

Subjects were recruited for this observational study from Kobe University Hospital (Kobe, Japan). The study protocol was approved by the institutional review board of Kobe University and adhered to the tenets of the Declaration of Helsinki. Written informed consent was obtained from each subject after explanation of the study.

Four patients with unilateral TON received full ocular examinations repeatedly for 8 months after the trauma. All patients had a history of indirect TON without any intraocular disease and any blowout orbital or optic canal fracture. All patients showed acute and severe visual loss with relative afferent pupillary defects in the affected eyes. The affected eyes did not have other ocular diseases and their intraocular pressure was normal. The contralateral eyes did not display ocular disease or provide abnormal visual field findings. The patient characteristics and visual outcomes are summarized in the Table.

Table Graphic Jump LocationTable. Parameters at the Final Examination

RTVue-100 OCT (software version 4.0.5.39) was used to observe retinal structural changes after TON. The optic nerve head map protocol was applied to evaluate the cpRNFL. This protocol generates a cpRNFL-thickness map based on measurements obtained along a circle 3.45 mm in diameter centered on the optic disc. The GCC protocol was used to determine the GCC and macular thickness. Only high-quality images, as defined by a signal strength index more than 30, were accepted. The first examination was performed at 1 week after injury. The subsequent examinations were performed at 2, 3, 4, 12, and 20 weeks after injury as well as at the final visit (32 to 36 weeks after injury). The average cpRNFL thickness and average GCC and macular thickness were evaluated at each examination. Figure 1 demonstrated the changes in cpRNFL thickness and in the GCC in case 1 over time.

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Figure 1. Changes in the optic nerve head map (A) and ganglion cell complex significance (B) of the right eye in case 1. Average circumpapillary retinal nerve fiber layer thickness and ganglion cell complex thickness in each session are also shown. IN indicates inferior nasal; IT, inferior temporal; NL, nasal lower; NT, nasal temporal; NU, nasal upper; SN, superior nasal; ST, superior temporal; T, temporal; TL, temporal lateral; and TU, temporal upper.

The means of these parameters in the contralateral eyes were averaged over 7 examinations and used as controls. The ratio of the affected eye measurement to the contralateral eye measurement was evaluated at each session.

Each individual showed a marked reduction in cpRNFL thickness and GCC in TON (Figure 2). The reduction in macular thickness was not as evident, as expected. The cpRNFL thickness and GCC were stable at 1 week after the injury but started to significantly decrease at 2 weeks after the injury (paired t test, cpRNFL, P = .01; GCC, P = .04). Macular thickness started to decrease significantly at 4 weeks after the injury (P = .01). The reduction occurred 2 to 8 weeks after the injury and had almost completely disappeared at 20 weeks. The cpRNFL measurements exhibited significantly greater reductions than did the GCC and macular thickness. The final relative ratio of cpRNFL thickness was 48%, 40%, 57%, and 62% in cases 1 through 4, respectively. The final rate of GCC was 64%, 68%, 71%, and 74% in cases 1 through 4, respectively. The final rate of macular thickness was 90%, 91%, 88%, and 89% in cases 1 through 4, respectively. Although visual acuity in case 3 recovered to 20/20, the relative rates were similar to those of other patients with severe vision loss.

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Figure 2. The time course of the mean (SD) ratio of the affected eyes to the contralateral eyes of circumpapillary retinal nerve fiber layer (cpRNFL) thickness, average ganglion cell complex (GCC), and macular thickness in 4 eyes after traumatic optic neuropathy. *Paired t test, P < .05 compared with the contralateral eyes.

Given that it is impossible to longitudinally perform histological examinations in humans, in vivo imaging is a valuable technique. Previous studies have explored reductions in cpRNFL thickness after TON. Three studies using scanning laser polarimetry reported that cpRNFL thickness did not decrease within 2 weeks after injury.13 On the contrary, 2 studies using time-domain OCT demonstrated that the cpRNFL returns to normal thickness within 1 week. Cunha et al4 showed that the cpRNFL thickness started to decrease 2 weeks after injury.5 This was consistent with our study. This discrepancy between scanning laser polarimetry and OCT in the reported onset of reduction in thickness might be related to the technology used.

Macular thickness (as measured with time-domain OCT) also started to significantly decrease at 4 weeks after injury, which was in agreement with previous studies.4,6 In addition, progressive thinning of the GCC was measured using spectral-domain OCT in this study. The GCC loss was greater than macular thickness loss at the final examination (paired t test, P < .001). The GCC reflects structural changes in the inner retina, including the retinal ganglion cells, more accurately than measurements of total macular thickness, which include the outer retinal architecture. The time course of the reduction in GCC thickness was similar to that observed for the cpRNFL. Therefore, the losses of retinal ganglion cells and related axons continue at similar rates as axonal injury. Technological developments that allow for dissociation of the retinal nerve fiber layer from the retinal ganglion cell layer will provide further information.

The current study demonstrated that the reductions in cpRNFL thickness and GCC started to decrease at 2 weeks after trauma and plateaued at 20 weeks in all cases. Certain treatments for TON should be available in the future, but these treatments should be performed by 20 weeks. This study also showed that fragments of the cpRNFL and GCC remained even in eyes that were completely blind. This result might be important during the monitoring of other optic neuropathies such as glaucoma.

Correspondence: Dr Kanamori, Division of Ophthalmology, Department of Surgery, Kobe University Graduate School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan (kanaaki@med.kobe-u.ac.jp).

Financial Disclosure: None reported.

Funding/Support: Supported by grants-in-aid 22390324 (Drs Negi, Yamada, and Nakamura) and 23791983 (Dr Kanamori) for scientific research by the Ministry of Education, Culture, Sports, and Science and Technology of the Japanese Government and Suda Memorial Foundation (Dr Kanamori).

Medeiros FA, Susanna R Jr. Retinal nerve fiber layer loss after traumatic optic neuropathy detected by scanning laser polarimetry.  Arch Ophthalmol. 2001;119(6):920-921
PubMed   |  Link to Article
Meier FM, Bernasconi P, Stürmer J, Caubergh MJ, Landau K. Axonal loss from acute optic neuropathy documented by scanning laser polarimetry.  Br J Ophthalmol. 2002;86(3):285-287
PubMed   |  Link to Article
Miyahara T, Kurimoto Y, Kurokawa T, Kuroda T, Yoshimura N. Alterations in retinal nerve fiber layer thickness following indirect traumatic optic neuropathy detected by nerve fiber analyzer, GDx-N.  Am J Ophthalmol. 2003;136(2):361-364
PubMed   |  Link to Article
Cunha LP, Costa-Cunha LV, Malta RF, Monteiro ML. Comparison between retinal nerve fiber layer and macular thickness measured with OCT detecting progressive axonal loss following traumatic optic neuropathy.  Arq Bras Oftalmol. 2009;72(5):622-625
PubMed   |  Link to Article
Medeiros FA, Moura FC, Vessani RM, Susanna R Jr. Axonal loss after traumatic optic neuropathy documented by optical coherence tomography.  Am J Ophthalmol. 2003;135(3):406-408
PubMed   |  Link to Article
Vessani RM, Cunha LP, Monteiro ML. Progressive macular thinning after indirect traumatic optic neuropathy documented by optical coherence tomography.  Br J Ophthalmol. 2007;91(5):697-698
PubMed   |  Link to Article

Figures

Place holder to copy figure label and caption
Graphic Jump Location

Figure 1. Changes in the optic nerve head map (A) and ganglion cell complex significance (B) of the right eye in case 1. Average circumpapillary retinal nerve fiber layer thickness and ganglion cell complex thickness in each session are also shown. IN indicates inferior nasal; IT, inferior temporal; NL, nasal lower; NT, nasal temporal; NU, nasal upper; SN, superior nasal; ST, superior temporal; T, temporal; TL, temporal lateral; and TU, temporal upper.

Place holder to copy figure label and caption
Graphic Jump Location

Figure 2. The time course of the mean (SD) ratio of the affected eyes to the contralateral eyes of circumpapillary retinal nerve fiber layer (cpRNFL) thickness, average ganglion cell complex (GCC), and macular thickness in 4 eyes after traumatic optic neuropathy. *Paired t test, P < .05 compared with the contralateral eyes.

Tables

Table Graphic Jump LocationTable. Parameters at the Final Examination

References

Medeiros FA, Susanna R Jr. Retinal nerve fiber layer loss after traumatic optic neuropathy detected by scanning laser polarimetry.  Arch Ophthalmol. 2001;119(6):920-921
PubMed   |  Link to Article
Meier FM, Bernasconi P, Stürmer J, Caubergh MJ, Landau K. Axonal loss from acute optic neuropathy documented by scanning laser polarimetry.  Br J Ophthalmol. 2002;86(3):285-287
PubMed   |  Link to Article
Miyahara T, Kurimoto Y, Kurokawa T, Kuroda T, Yoshimura N. Alterations in retinal nerve fiber layer thickness following indirect traumatic optic neuropathy detected by nerve fiber analyzer, GDx-N.  Am J Ophthalmol. 2003;136(2):361-364
PubMed   |  Link to Article
Cunha LP, Costa-Cunha LV, Malta RF, Monteiro ML. Comparison between retinal nerve fiber layer and macular thickness measured with OCT detecting progressive axonal loss following traumatic optic neuropathy.  Arq Bras Oftalmol. 2009;72(5):622-625
PubMed   |  Link to Article
Medeiros FA, Moura FC, Vessani RM, Susanna R Jr. Axonal loss after traumatic optic neuropathy documented by optical coherence tomography.  Am J Ophthalmol. 2003;135(3):406-408
PubMed   |  Link to Article
Vessani RM, Cunha LP, Monteiro ML. Progressive macular thinning after indirect traumatic optic neuropathy documented by optical coherence tomography.  Br J Ophthalmol. 2007;91(5):697-698
PubMed   |  Link to Article

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