Submitted for Publication: September 3, 2012; final revision received November 5, 2012; accepted November 7, 2012.
Published Online: April 18, 2013. 10.1001/jamaophthalmol.2013.2385. Corrected May 2, 2013.
Author Contributions: Drs Schmidt and D. A. Sullivan are co–first authors.
Conflict of Interest Disclosures: Drs Schmidt, D. A. Sullivan, and B. D. Sullivan are cofounders of a company, Singularis, that was designed to develop a recombinant human lubricin protein for the treatment of dry eye disease and other health care conditions. Singularis has merged with Lubris, which retains the same objectives. A series of patents have been awarded or filed around this technology. The intellectual property for the dry eye application is owned by the Schepens Eye Research Institute/Massachusetts Eye and Ear Infirmary and the University of California, San Diego.
Funding/Support: This research was supported by National Institutes of Health grant R01EY05612, the Margaret S. Sinon Scholar in Ocular Surface Research fund, and funding from the Natural Sciences and Engineering Research Council of Canada, the Canadian Arthritis Network, and the Faculty of Kinesiology and the Schulich School of Engineering Centre for Bioengineering Research and Education at the University of Calgary. We acknowledge use of tissues procured by the National Disease Research Interchange with support from National Institutes of Health grant 5 U42 RR006042.
Additional Contributions: We express our appreciation to Zahra Sadrai, MD (Boston, Massachusetts), for mouse cornea and conjunctival RNA preparations; Roman Krawetz, PhD (Calgary, Alberta, Canada), for human articular cartilage and synovial membrane complementary DNA samples; Pablo Argueso, PhD, and Abha Gulati, MD (Boston), for human conjunctival impression cytology complementary DNA samples; Ilene K. Gipson, PhD, and Sandra Michaud, MS (Boston), for immortalized human conjunctival epithelial cells; James Jester, PhD (Irvine, California), for immortalized human corneal epithelial cells; Matthew L. Warman, MD (Boston), and Yajun Cui, PhD (Cleveland, Ohio), for making available breeding pairs of mice with a 129Sv/Ev genetic background and Ms Stefanie DeLuca (Wilmington, Massachusetts) for help with these animals; Prof M. Dietel and Dr M. von Laffert (Berlin, Germany) for providing human articular tissues; the Lions Eye Bank through the Southern Alberta Organ and Tissue Donation Program (Calgary) for providing human corneas; and the University of Calgary Body Donation Program (Calgary) for providing human eyelids.