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Original Investigation |

Prolonged Curvularia Endophthalmitis Due to Organism Sequestration FREE

Aleksandra V. Rachitskaya, MD1; Ashvini K. Reddy, MD1,2; Darlene Miller, DHSc, MPH1; Janet Davis, MD1; Harry W. Flynn Jr, MD1; William Smiddy, MD1; Wilfredo Lara, MD3; Selina Lin, MD4; Sander Dubovy, MD1; Thomas A. Albini, MD1
[+] Author Affiliations
1Bascom Palmer Eye Institute, Department of Ophthalmology, Miller School of Medicine, University of Miami, Miami, Florida
2Department of Ophthalmology, University of Virginia School of Medicine, Charlottesville
3Retina Macula Specialists of Miami, Miami, Florida
4Kaufman Eye Institute, Zephyrhills, Florida
JAMA Ophthalmol. 2014;132(9):1123-1126. doi:10.1001/jamaophthalmol.2014.1069.
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Published online

Importance  Endophthalmitis caused by Curvularia is a rare condition seen after cataract surgery and trauma. The clinical course has not been described previously.

Objective  To examine the clinical course of 6 postoperative and trauma-related cases of Curvularia endophthalmitis.

Design, Setting, and Participants  Retrospective case series. We reviewed the archives of the microbiology laboratory of Bascom Palmer Eye Institute, a tertiary referral hospital, from January 1, 1980, through September 30, 2013, to identify cases of Curvularia endophthalmitis. Data collected included demographic information, the cause of endophthalmitis, presenting features, treatment course, the number of recurrences, the area of organism sequestration, and final visual outcome.

Exposures  Trauma and cataract surgery.

Main Outcomes and Measures  Times from the inciting event to presentation of symptoms, diagnosis, and eradication; visual acuity; and identification of the area of sequestration.

Results  We identified 6 patients with Curvularia endophthalmitis, including 5 who underwent cataract surgery and 1 after trauma. The diagnosis was established rapidly in the trauma case. In the postoperative cases, the time from the surgery to first symptoms ranged from 2 to 5 months; from the surgery to correct diagnosis, 7 to 24 months; and from the surgery to eradication, 8 to 27 months. Despite aggressive antifungal therapy, eradication of the infection could be achieved only by identification and removal of the nidus of sequestration. The median follow-up was 29.5 months.

Conclusions and Relevance  In cases of endophthalmitis caused by Curvularia, the diagnosis and treatment are often delayed, especially in postoperative cases. The eradication of the organism requires identification and removal of the nidi of sequestration.

Figures in this Article

Curvularia is a dematiaceous or darkly pigmented fungus. Endophthalmitis due to Curvularia species is exceedingly rare, with only isolated case reports in the literature.16 Large series of endogenous and exogenous fungal endophthalmitis in the United States do not mention Curvularia as a separate causative organism.710 The present series of 6 cases of Curvularia endophthalmitis demonstrates the difficulty of timely and proper diagnosis and challenges in the eradication of the microorganism.

An institutional review board–approved retrospective review of the archives of the microbiology laboratory at Bascom Palmer Eye Institute from January 1, 1980, through September 30, 2013, identified all cultures positive for Curvularia. The substrate (blood agar, chocolate agar, Sabouraud agar, Lowenstein-Jensen agar, or thioglycolate broth) on which Curvularia grew was documented with the time from initial specimen plating to organism growth. We identified a total of 7 patients with vitreous cultures positive for Curvularia. One additional patient had culture-confirmed Curvularia endophthalmitis with a vitreous culture performed at another hospital. Because the patient was treated for her condition at Bascom Palmer Eye Institute and we had access to the patient’s complete medical records, she was included in the study. Thus, we reviewed the records of 8 patients. Two patients were excluded after review of the clinical history because they did not develop clinical endophthalmitis. Therefore, we included a total of 6 cases of Curvularia endophthalmitis, with 2 of these patients previously described in separate reports4,5 (cases 1 and 2 in the Supplement [eAppendix]).

A total of 6 patients with endophthalmitis caused by Curvularia species were identified and reviewed in chronological order (Table). In 5 patients (mean age, 75.2 years), endophthalmitis occurred after cataract surgery with intraocular lens implantation; in 1 patient (age, 21 years), endophthalmitis occurred after trauma. The diagnosis was established rapidly at 6 weeks in the trauma case. In the postoperative cases, the time from surgery to the first signs of inflammation ranged from 2 to 5 months. The time from the surgery to correct diagnosis of Curvularia endophthalmitis ranged from 7 to 24 months. The patients with postoperative endophthalmitis were treated with topical corticosteroids (n = 5), sub–Tenon or subconjunctival injection of triamcinolone acetonide (n = 3), or systemic methotrexate sodium and azathioprine sodium (n = 1). These patients underwent a mean of 2 (range, 0-5) intraocular procedures and operations, including anterior chamber culture, vitreous culture, intraocular injection, or pars plana vitrectomy. In 5 postoperative cases, the definitive surgery that established the diagnosis of Curvularia endophthalmitis was pars plana vitrectomy with vitreous cultures, partial or complete capsulectomy or lensectomy, and intravitreal injection of antimicrobials. The time from the inciting event (surgery or trauma) to eradication in all 6 patients ranged from 8 to 27 months. During this time the patients were treated with topical, oral, and, in 1 case, intravenous antifungals. They underwent a mean of 1.8 intraocular procedures, usually with concurrent intravitreal antifungal treatment, with a mean of 6.8 separate intravitreal antifungal injections. Despite aggressive antifungal therapy, eradication of the infection could be achieved only by identification and removal of the nidus of sequestration. This process required the use of imaging studies, such as ultrasonography, and use of endoscopy during the pars plana vitrectomy. The nidi of sequestration included an iris lesion, a corneal endothelial plaque, ciliary body membranes, capsular bag, and subretinal abscess (several of these nidi are shown in the Figure). The visual acuity at presentation ranged from 20/70 to hand motions. The median follow-up was 29.5 (range, 11-35) months. The final visual acuity ranged from 20/60 to light perception.

Table Graphic Jump LocationTable.  Clinical Characteristics of Patients With Curvularia Endophthalmitis
Place holder to copy figure label and caption
Figure.
Nidi of Sequestration in Curvularia Endophthalmitis

A, A hypopyon and an iris lesion, representing a nidus of Curvularia sequestration, in a patient with pseudophakia (case 1). B, Corneal laceration repair with corneal infiltrate harboring Curvularia organisms in a trauma patient (case 2). C, Histopathologic photomicrograph of Curvularia organisms in the corneal stroma of the trauma patient (Gomori methenamine silver, original magnification ×100) (case 2). D, Posterior capsular opacity and Curvularia endophthalmitis in a patient with pseudophakia (case 3).

Graphic Jump Location

We report the first, to our knowledge, clinical series (6 patients) with intraocular cultures positive for Curvularia species. Curvularia, a widespread septate mold that is black owing to melanin in its hyphae and conidia, is found in tropical climates, lives in the soil and on plants, and is spread by airborne spores.11 In the literature, the number of reports of Curvularia keratitis has increased through the last half of the 20th century, with most of the reports coming from India, the United States, and Asia.11 Nevertheless, fungal keratitis due to Curvularia is less frequent than that due to Candida, Fusarium, or Aspergillus species.11 Cases of Curvularia endophthalmitis are rare, but reports are increasing. The first reports of Curvularia endophthalmitis are from India in early 2000.3,6,12 Review of the records of the microbiology laboratory at our institution disclosed that the first case of clinically significant Curvularia endophthalmitis was documented in 2006 (case 1; Table). Four large studies focusing on endogenous and exogenous fungal endophthalmitis at our institution spanning 1984 through 199310 and 1990 through 20098 for endogenous and 1969 through 19867 and 1990 through 20069 for exogenous infections did not identify any cases of Curvularia endophthalmitis.

Curvularia was not documented as a human pathogen until 1959.13Curvularia species most commonly cause skin infections, invasive sinusitis, and allergic fungal sinusitis.14,15 Cases of pulmonary and cerebral infection and arthritis, osteomyelitis, fungemia, endocarditis, peritonitis, and gastrointestinal tract disease have been reported.14,1618 Infections with Curvularia do not appear to require an immunosuppressed host, and inhalation and skin inoculation are the main routes of infection.15,18

In the present study, 1 patient was otherwise healthy (case 2). The other patients had several comorbidities such as hypertension, diabetes mellitus, cerebral aneurysm, stroke, anemia, hypothyroidism, and possible rheumatoid arthritis (Table). However, none of them were significantly immunocompromised.

In Curvularia keratitis, trauma with plant matter or dirt is the leading cause of infection.11 In the present trauma-related case of Curvularia endophthalmitis, the trauma was due to nail injury. No unusual exposure was seen in surgery-related cases. However, Curvularia species have been isolated in the conjunctival sac of healthy individuals.19 In the trauma-related case of endophthalmitis, the diagnosis was established rapidly because the signs suggestive of infectious endophthalmitis increased the level of suspicion. The patients with Curvularia endophthalmitis after cataract surgery had delayed diagnosis and appropriate therapy. The time from surgery to first symptoms ranged from 2 to 5 months. The time from surgery to correct diagnosis ranged from 7 to 24 months. In fact, 3 of 5 patients were treated with sub-Tenon or subconjunctival injection of corticosteroids in addition to topical corticosteroid application before the appropriate diagnosis, and 1 patient received oral immunosuppressive therapy. Propionibacterium acnes is the most common causative organism of delayed-onset endophthalmitis and presents with similar low-grade inflammation and a slowly progressive course; a posterior capsular plaque is present in about 65% of patients.20 In Curvularia endophthalmitis, the plaque was seen in 3 of 5 patients who had undergone surgery for cataract. Compared with P acnes, Curvularia infection presented with a variety of locations of sequestration, including an endothelial corneal plaque, the posterior lens capsule, a vitreous fungus ball, membranes on the ciliary body, and the subretinal space.

In the present case series, the patients received an aggressive treatment consisting of combined intravitreal, oral, topical, and occasionally intravenous antifungals and surgical intervention. Diagnostic and therapeutic vitrectomy was necessary in all cases. All the reported cases clearly demonstrate the importance of identification and elimination of the possible source of sequestered infection. This process may require use of preoperative high-resolution ultrasonography and thorough intraoperative depression or endoscopy to visualize the retinal periphery and the retroirideal space.

Curvularia infection represents a rare cause of endophthalmitis. The diagnosis and treatment are often delayed, especially in the postoperative cases. The eradication of the organism requires identification and removal of the nidus of sequestration.

Submitted for Publication: November 20, 2013; final revision received February 17, 2014; accepted February 20, 2014.

Corresponding Author: Thomas A. Albini, MD, Bascom Palmer Eye Institute, Department of Ophthalmology, Miller School of Medicine, University of Miami, 900 NW 17th St, Miami, FL 33136 (talbini@med.miami.edu).

Published Online: June 5, 2014. doi:10.1001/jamaophthalmol.2014.1069.

Author Contributions: Dr Albini had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.

Study concept and design: Rachitskaya, Flynn, Albini.

Acquisition, analysis, or interpretation of data: All authors.

Drafting of the manuscript: Rachitskaya, Reddy, Lara, Lin.

Critical revision of the manuscript for important intellectual content: Rachitskaya, Miller, Davis, Flynn, Smiddy, Lin, Dubovy, Albini.

Statistical analysis: Flynn.

Administrative, technical, or material support: Rachitskaya, Reddy, Miller, Lara, Albini.

Study supervision: Dubovy, Albini.

Conflict of Interest Disclosures: None reported.

Funding/Support: This study is supported in part by Center Core Grant P30EY014801 from the National Institutes of Health; by an unrestricted grant from Research to Prevent Blindness; and by grant W81XWH-09-1-0675 from the Department of Defense.

Role of the Sponsor: The funding sources had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.

Previous Presentations: This paper was presented at the Annual Meeting of the Association for Research in Vision and Ophthalmology; May 5, 2013; Seattle, Washington; at the Annual Meeting of the American Society of Retina Specialists; August 25, 2013; Toronto, Ontario, Canada; and at the 47th Annual Meeting of the Ocular Microbiology and Immunology Group; November 15, 2013; New Orleans, Louisiana.

Ehlers  JP, Chavala  SH, Woodward  JA, Postel  EA.  Delayed recalcitrant fungal endophthalmitis secondary to CurvulariaCan J Ophthalmol.2011;46(2):199-200.
PubMed   |  Link to Article
Berbel  RF, Casella  AM, de Freitas  D, Höfling-Lima  AL.  Curvularia lunata endophthalmitis. J Ocul Pharmacol Ther.2011;27(5):535-537.
PubMed   |  Link to Article
Kaushik  S, Ram  J, Chakrabarty  A, Dogra  MR, Brar  GS, Gupta  A.  Curvularia lunata endophthalmitis with secondary keratitis. Am J Ophthalmol. 2001;131(1):140-142.
PubMed   |  Link to Article
Pathengay  A, Miller  DM, Flynn  HW  Jr, Dubovy  SR.  Curvularia endophthalmitis following open globe injuries. Arch Ophthalmol. 2012;130(5):652-654.
PubMed   |  Link to Article
Sisk  RASW, Dubovy  SR, Miller  D.  Chronic Curvularia lunata endophthalmitis following cataract extraction. Retinal Cases Brief Rep.2009;3(4):438-439.
Link to Article
Pathengay  A, Shah  GY, Das  T, Sharma  S.  Curvularia lunata endophthalmitis presenting with a posterior capsular plaque. Indian J Ophthalmol. 2006;54(1):65-66.
PubMed   |  Link to Article
Pflugfelder  SC, Flynn  HW  Jr, Zwickey  TA,  et al.  Exogenous fungal endophthalmitis. Ophthalmology. 1988;95(1):19-30.
PubMed   |  Link to Article
Lingappan  A, Wykoff  CC, Albini  TA,  et al.  Endogenous fungal endophthalmitis: causative organisms, management strategies, and visual acuity outcomes. Am J Ophthalmol.2012;153(1):162-166.e1. doi:10.1016/j.ajo.2011.06.020.
PubMed   |  Link to Article
Wykoff  CC, Flynn  HW  Jr, Miller  D, Scott  IU, Alfonso  EC.  Exogenous fungal endophthalmitis: microbiology and clinical outcomes. Ophthalmology.2008;115(9):1501-1507.e2. doi:10.1016/j.ophtha.2008.02.027.
PubMed   |  Link to Article
Essman  TF, Flynn  HW  Jr, Smiddy  WE,  et al.  Treatment outcomes in a 10-year study of endogenous fungal endophthalmitis. Ophthalmic Surg Lasers. 1997;28(3):185-194.
PubMed
Wilhelmus  KR, Jones  DB.  Curvularia keratitis. Trans Am Ophthalmol Soc.2001;99:111-132.
PubMed
Chakrabarti  A, Shivaprakash  MR, Singh  R,  et al.  Fungal endophthalmitis: fourteen years’ experience from a center in India. Retina. 2008;28(10):1400-1407.
PubMed   |  Link to Article
Baylet  J, Camain  R, Segretain  G.  Identification of the agents of maduromycoses of Senegal and Mauritania: description of a new species [in French]. Bull Soc Pathol Exot Filiales. 1959;52:448-477.
PubMed
Silveira  F, Nucci  M.  Emergence of black moulds in fungal disease: epidemiology and therapy. Curr Opin Infect Dis. 2001;14(6):679-684.
PubMed   |  Link to Article
Brandt  ME, Warnock  DW.  Epidemiology, clinical manifestations, and therapy of infections caused by dematiaceous fungi. J Chemother. 2003;15(suppl 2):36-47.
PubMed   |  Link to Article
Varughese  S, David  VG, Mathews  MS, Tamilarasi  V.  A patient with amphotericin-resistant Curvularia lunata peritonitis. Perit Dial Int.2011;31(1):108-109.
PubMed   |  Link to Article
Berg  D, Garcia  JA, Schell  WA, Perfect  JR, Murray  JC.  Cutaneous infection caused by Curvularia pallescens: a case report and review of the spectrum of disease. J Am Acad Dermatol. 1995;32(2, pt 2):375-378.
PubMed   |  Link to Article
Carter  E, Boudreaux  C.  Fatal cerebral phaeohyphomycosis due to Curvularia lunata in an immunocompetent patient. J Clin Microbiol. 2004;42(11):5419-5423.
PubMed   |  Link to Article
Sehgal  SC, Dhawan  S, Chhiber  S, Sharma  M, Talwar  P.  Frequency and significance of fungal isolations from conjunctival sac and their role in ocular infections. Mycopathologia. 1981;73(1):17-19.
PubMed   |  Link to Article
Shirodkar  AR, Pathengay  A, Flynn  HW  Jr,  et al.  Delayed- versus acute-onset endophthalmitis after cataract surgery. Am J Ophthalmol.2012;153(3):391-398.e2. doi:10.1016/j.ajo.2011.08.029.
PubMed   |  Link to Article

Figures

Place holder to copy figure label and caption
Figure.
Nidi of Sequestration in Curvularia Endophthalmitis

A, A hypopyon and an iris lesion, representing a nidus of Curvularia sequestration, in a patient with pseudophakia (case 1). B, Corneal laceration repair with corneal infiltrate harboring Curvularia organisms in a trauma patient (case 2). C, Histopathologic photomicrograph of Curvularia organisms in the corneal stroma of the trauma patient (Gomori methenamine silver, original magnification ×100) (case 2). D, Posterior capsular opacity and Curvularia endophthalmitis in a patient with pseudophakia (case 3).

Graphic Jump Location

Tables

Table Graphic Jump LocationTable.  Clinical Characteristics of Patients With Curvularia Endophthalmitis

References

Ehlers  JP, Chavala  SH, Woodward  JA, Postel  EA.  Delayed recalcitrant fungal endophthalmitis secondary to CurvulariaCan J Ophthalmol.2011;46(2):199-200.
PubMed   |  Link to Article
Berbel  RF, Casella  AM, de Freitas  D, Höfling-Lima  AL.  Curvularia lunata endophthalmitis. J Ocul Pharmacol Ther.2011;27(5):535-537.
PubMed   |  Link to Article
Kaushik  S, Ram  J, Chakrabarty  A, Dogra  MR, Brar  GS, Gupta  A.  Curvularia lunata endophthalmitis with secondary keratitis. Am J Ophthalmol. 2001;131(1):140-142.
PubMed   |  Link to Article
Pathengay  A, Miller  DM, Flynn  HW  Jr, Dubovy  SR.  Curvularia endophthalmitis following open globe injuries. Arch Ophthalmol. 2012;130(5):652-654.
PubMed   |  Link to Article
Sisk  RASW, Dubovy  SR, Miller  D.  Chronic Curvularia lunata endophthalmitis following cataract extraction. Retinal Cases Brief Rep.2009;3(4):438-439.
Link to Article
Pathengay  A, Shah  GY, Das  T, Sharma  S.  Curvularia lunata endophthalmitis presenting with a posterior capsular plaque. Indian J Ophthalmol. 2006;54(1):65-66.
PubMed   |  Link to Article
Pflugfelder  SC, Flynn  HW  Jr, Zwickey  TA,  et al.  Exogenous fungal endophthalmitis. Ophthalmology. 1988;95(1):19-30.
PubMed   |  Link to Article
Lingappan  A, Wykoff  CC, Albini  TA,  et al.  Endogenous fungal endophthalmitis: causative organisms, management strategies, and visual acuity outcomes. Am J Ophthalmol.2012;153(1):162-166.e1. doi:10.1016/j.ajo.2011.06.020.
PubMed   |  Link to Article
Wykoff  CC, Flynn  HW  Jr, Miller  D, Scott  IU, Alfonso  EC.  Exogenous fungal endophthalmitis: microbiology and clinical outcomes. Ophthalmology.2008;115(9):1501-1507.e2. doi:10.1016/j.ophtha.2008.02.027.
PubMed   |  Link to Article
Essman  TF, Flynn  HW  Jr, Smiddy  WE,  et al.  Treatment outcomes in a 10-year study of endogenous fungal endophthalmitis. Ophthalmic Surg Lasers. 1997;28(3):185-194.
PubMed
Wilhelmus  KR, Jones  DB.  Curvularia keratitis. Trans Am Ophthalmol Soc.2001;99:111-132.
PubMed
Chakrabarti  A, Shivaprakash  MR, Singh  R,  et al.  Fungal endophthalmitis: fourteen years’ experience from a center in India. Retina. 2008;28(10):1400-1407.
PubMed   |  Link to Article
Baylet  J, Camain  R, Segretain  G.  Identification of the agents of maduromycoses of Senegal and Mauritania: description of a new species [in French]. Bull Soc Pathol Exot Filiales. 1959;52:448-477.
PubMed
Silveira  F, Nucci  M.  Emergence of black moulds in fungal disease: epidemiology and therapy. Curr Opin Infect Dis. 2001;14(6):679-684.
PubMed   |  Link to Article
Brandt  ME, Warnock  DW.  Epidemiology, clinical manifestations, and therapy of infections caused by dematiaceous fungi. J Chemother. 2003;15(suppl 2):36-47.
PubMed   |  Link to Article
Varughese  S, David  VG, Mathews  MS, Tamilarasi  V.  A patient with amphotericin-resistant Curvularia lunata peritonitis. Perit Dial Int.2011;31(1):108-109.
PubMed   |  Link to Article
Berg  D, Garcia  JA, Schell  WA, Perfect  JR, Murray  JC.  Cutaneous infection caused by Curvularia pallescens: a case report and review of the spectrum of disease. J Am Acad Dermatol. 1995;32(2, pt 2):375-378.
PubMed   |  Link to Article
Carter  E, Boudreaux  C.  Fatal cerebral phaeohyphomycosis due to Curvularia lunata in an immunocompetent patient. J Clin Microbiol. 2004;42(11):5419-5423.
PubMed   |  Link to Article
Sehgal  SC, Dhawan  S, Chhiber  S, Sharma  M, Talwar  P.  Frequency and significance of fungal isolations from conjunctival sac and their role in ocular infections. Mycopathologia. 1981;73(1):17-19.
PubMed   |  Link to Article
Shirodkar  AR, Pathengay  A, Flynn  HW  Jr,  et al.  Delayed- versus acute-onset endophthalmitis after cataract surgery. Am J Ophthalmol.2012;153(3):391-398.e2. doi:10.1016/j.ajo.2011.08.029.
PubMed   |  Link to Article

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