0
We're unable to sign you in at this time. Please try again in a few minutes.
Retry
We were able to sign you in, but your subscription(s) could not be found. Please try again in a few minutes.
Retry
There may be a problem with your account. Please contact the AMA Service Center to resolve this issue.
Contact the AMA Service Center:
Telephone: 1 (800) 262-2350 or 1 (312) 670-7827  *   Email: subscriptions@jamanetwork.com
Error Message ......
Research Letter |

Loop-Mediated Isothermal Amplification for Rapid Diagnosis of Tubercular Uveitis

Kusum Sharma, MD1; Reema Bansal, MD2; Aman Sharma, MD3; Nikhil Beke, MD2; Amod Gupta, MD2
[+] Author Affiliations
1Department of Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
2Department of Ophthalmology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
3Department of Internal Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh, India
JAMA Ophthalmol. 2014;132(6):777-778. doi:10.1001/jamaophthalmol.2014.330.
Text Size: A A A
Published online

Extract

Polymerase chain reaction (PCR) to detect Mycobacterium tuberculosis in tubercular uveitis is limited by low sensitivity, expensive equipment, sophisticated laboratory facilities, and expertise. We evaluated loop-mediated isothermal amplification (LAMP) assay1 targeting the IS6110 region of M tuberculosis in intraocular samples from patients with tubercular uveitis.

Article InformationCorresponding Author: Amod Gupta, MD, Department of Ophthalmology, Post Graduate Institute of Medical Education and Research, Chandigarh, India 160012 (eyepgi@sify.com).

Author Contributions: Dr K. Sharma had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.

Study concept and design: K. Sharma, A. Sharma, Gupta.

Acquisition, analysis, or interpretation of data: K. Sharma, Bansal, A. Sharma, Beke.

Drafting of the manuscript: K. Sharma, Bansal, A. Sharma, Beke.

Critical revision of the manuscript for important intellectual content: K. Sharma, Gupta.

Statistical analysis: K. Sharma.

Administrative, technical, or material support: A. Sharma, Gupta.

Study supervision: Bansal, Gupta.

Conflict of Interest Disclosures: None reported.

Figures in this Article

Sign in

Create a free personal account to sign up for alerts, share articles, and more.

Purchase Options

• Buy this article
• Subscribe to the journal

First Page Preview

View Large
First page PDF preview

First Page Preview

View Large
First page PDF preview

Figures

Place holder to copy figure label and caption
Figure 1.
Results of Loop-Mediated Isothermal Amplification by Agarose Gel–Based Analysis Showing a Typical Ladder Pattern in a Positive Reaction

Lane 1 (L1) is a 100–base pair molecular marker; L2, positive control with bending pattern on gel; L3 and L4, positive clinical samples; L5, negative control; and L6 and L7, negative clinical samples.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 2.
Results of Loop-Mediated Isothermal Amplification Using 0.1% SYBR Green 1 (Life Technologies) as Seen by Naked Eye

Tube 1 (T1) is a positive control (green color); T2 through T6, clinical samples with positive amplification; T7, negative control (SYBR Green I dye with no change in color); and T8 through T10, negative clinical samples.

Graphic Jump Location

Tables

References

Correspondence

CME
Also Meets CME requirements for:
Browse CME for all U.S. States
Accreditation Information
The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity. Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
Your answers have been saved for later.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
Commitment to Change (optional):
Indicate what change(s) you will implement in your practice, if any, based on this CME course.
Your quiz results:
The filled radio buttons indicate your responses. The preferred responses are highlighted
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.
Submit a Comment

Multimedia

Some tools below are only available to our subscribers or users with an online account.

Sign in

Create a free personal account to sign up for alerts, share articles, and more.

Purchase Options

• Buy this article
• Subscribe to the journal

Related Content

Customize your page view by dragging & repositioning the boxes below.

Articles Related By Topic
Related Collections
PubMed Articles
Jobs
brightcove.createExperiences();