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Comment & Response |

Loop-Mediated Isothermal Amplification for Rapid Diagnosis of Tubercular Uveitis—Reply

Kusum Sharma, MBBS, MD1; Aman Sharma, MBBS, MD2; Amod Gupta, MD3
[+] Author Affiliations
1Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
2Department of Internal Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India
3Department of Ophthalmology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
JAMA Ophthalmol. 2015;133(2):226. doi:10.1001/jamaophthalmol.2014.4261.
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In Reply We appreciate the clarification by Balne and colleagues. Their article did indeed appear in November 2013.1 Perhaps we should have emphasized that before their article had been published, we had presented the content of our later-published work as a peer-reviewed poster at the 2012 Annual Meeting of the American Academy of Ophthalmology in Chicago, Illinois, on November 12, 2012.2 Of note, Balne and colleagues compared their LAMP assay with multiplex PCR results published by our group.3 Second, they raised the issue of our choosing IS6110 as a target leading to the LAMP assay missing it in 3 samples. While a higher sensitivity of LAMP assay using MPB64 can only be shown in a large prospective study, to our knowledge, IS6110 is the most commonly used target owing to its multiple copy numbers in the Mycobacterium genome.

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February 1, 2015
Praveen Kumar Balne, MSc; Soumyava Basu, MS; Savitri Sharma, MD
1Ocular Microbiology Service, L V Prasad Eye Institute, Bhubaneswar, India
2Retina-Vitreous Service, L V Prasad Eye Institute, Bhubaneswar, India3Uveitis Service, L V Prasad Eye Institute, Bhubaneswar, India
JAMA Ophthalmol. 2015;133(2):225-226. doi:10.1001/jamaophthalmol.2014.4257.
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