All animal experiments were conducted in accord with the University of Wisconsin Madison Medical School institutional animal care and use committees, with the National Institutes of Health guidelines, and with the Association for Research in Vision and Ophthalmology Statement on the Use of Animals in Ophthalmic and Vision Research. Specifically, simultaneous contralateral control injections with vehicle solutions presumed to be harmless are approved under this protocol. Three New Zealand white rabbits were anesthetized with intramuscular injections of ketamine hydrochloride (35-45 mg/kg), xylazine hydrochloride (5 mg/kg), and acepromazine maleate (0.75 mg/kg), followed by topical 0.5% proparacaine hydrochloride drops. Intracameral injections of 50 μL of 100% ethanol were made through a corneal paracentesis in 3 eyes, and an identical injection of the same volume of balanced salt solution was made in the contralateral eye to serve as a control. The rabbits received slitlamp examination on days 1, 2, 3, 6, 10, and 14 postinjection and were photographed on days 3 and 6 postinjection. The rabbits were humanely killed on day 14. The eyes were enucleated and fixed in 4% paraformaldehyde. Histological assessment was performed on the 3 treated eyes and 1 control eye.