We reviewed the clinical records of all patients of the Ocular Oncology Service at Wills Eye Hospital with the diagnosis of uveal melanoma who underwent FNAB for genetic testing for chromosome 3 status between November 1, 2005, and March 1, 2006. The Wills Eye Hospital institutional review board issued approval for this retrospective study. Data were gathered regarding clinical and genetic features of the tumor. The clinical data at initial examination included age, race (African American, Hispanic, Asian, or white), sex (female or male), affected eye (right or left), visual acuity, and symptoms. The tumor data included location (iris, ciliary body, or choroid), quadrant location (inferior, temporal, superior, nasal, or macula), anteroposterior location (macula, macula-equator, equator-ora, ciliary body, or iris), distance to the optic nerve (in millimeters), distance to the foveola (in millimeters), tumor basal dimension (in millimeters), tumor thickness (in millimeters by ultrasonography), subretinal fluid, orange pigment on the tumor surface, and previous documented tumor growth. The FNAB parameters included needle gauge, route (transscleral tumor base approach or transvitreal tumor apex approach), and fundus findings immediately after biopsy. Follow-up data included needle biopsy complications and recurrence at the site of biopsy.