0
We're unable to sign you in at this time. Please try again in a few minutes.
Retry
We were able to sign you in, but your subscription(s) could not be found. Please try again in a few minutes.
Retry
There may be a problem with your account. Please contact the AMA Service Center to resolve this issue.
Contact the AMA Service Center:
Telephone: 1 (800) 262-2350 or 1 (312) 670-7827  *   Email: subscriptions@jamanetwork.com
Error Message ......
Letters |

Difficulties in Mutation Screening of the Plasminogen (PLG) Gene in Patients With Ligneous Conjunctivitis and Severe Hypoplasminogenemia

Katrin Tefs, PhD; Louise Kobelt; Maike Ziegler; Boris Hügle, MD; Volker Schuster, MD
Arch Ophthalmol. 2007;125(9):1303. doi:10.1001/archopht.125.9.1303-a.
Text Size: A A A
Published online

Extract

Leyva-Castillo et al1 described a Mexican patient with ligneous conjunctivitis. Hemostaseologic studies revealed severe hypoplasminogenemia (type I plasminogen deficiency). Mutation screening of the plasminogen (PLG) gene (chromosome 6q26) suggested a 14–base pair deletion in exon 5 of the PLG gene (deleted sequence, GAACTACTGCAGGA). However, this deletion has already been described before as the result of a methodical error: accidental coamplification of sequences of the plasminogen-related gene B (NCBI accession number AC093616.5) located on chromosome 2.2,3 Furthermore, polymerase chain reaction amplification of PLG gene exons 1, 3, 4, 5, 13, 16, and 17 may lead to “false” distinct nucleotide exchanges owing to coamplified PLG homologous genes.3 Optimizing polymerase chain reaction conditions by, for example, increasing the annealing temperature or changing the primers will probably lead to the disappearance of the alleged deletion and false nucleotide exchanges.3,4 Another method to avoid coamplification of PLG homologous genes is a long polymerase chain reaction amplification strategy as described recently.5 In conclusion, the described amplified sequences are not the PLG gene and are possibly derived from PLG-like genes (PLGL) on chromosome 2, or the PLG gene of the patient has undergone a gene conversion with apolipoprotein(a) (LPA) on chromosome 6 or PLGL on chromosome 2.

Topics

Sign in

Purchase Options

• Buy this article
• Subscribe to the journal
• Rent this article ?

First Page Preview

View Large
First page PDF preview

Figures

Tables

References

Correspondence

CME
Also Meets CME requirements for:
Browse CME for all U.S. States
Accreditation Information
The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity. Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
Please click the checkbox indicating that you have read the full article in order to submit your answers.
Your answers have been saved for later.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
Commitment to Change (optional):
Indicate what change(s) you will implement in your practice, if any, based on this CME course.
Your quiz results:
The filled radio buttons indicate your responses. The preferred responses are highlighted
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.

Multimedia

Some tools below are only available to our subscribers or users with an online account.

52 Views
0 Citations
×

Sign in

Purchase Options

• Buy this article
• Subscribe to the journal
• Rent this article ?

Related Content

Customize your page view by dragging & repositioning the boxes below.

Jobs
brightcove.createExperiences();