Samples were air dried and fixed in acetone, followed by blocking in normal goat serum (dilution, 1:10). Samples were incubated with primary antibodies against desmoglein 3 (clone 5G11; dilution, 1:25; mouse; Zymed Laboratories, Inc, South San Francisco, California) and tenascin C (clone H-300; dilution, 1:250; rabbit; Santa Cruz Biotechnology, Inc, Santa Cruz, California) overnight at 4°C. The samples were washed and incubated with secondary antibodies Alexa Fluor 555 (antirabbit) and 488 (antimouse) (Invitrogen Ltd, Paisley, Scotland) for 1 hour. Samples were counterstained with propidium iodide and 4′,6-diamidino-2-phenylindole, mounted with glycerol mounting medium (Dako, Glostrup, Denmark), and examined under an Alphaphot-2 fluorescent microscope (Nikon UK, Surrey, England) or a DMRB microscope (Leica Microsystems Ltd). Images were either captured on a D70S digital camera (Nikon UK) using Camera Control software (Nikon UK) or a C4742-95 digital camera (Hamamatsu, Middlesex, England) using Openlab software (Improvision, Coventry, England). All of the images were edited on Photoshop CS2 software (Adobe Systems Inc, San Jose, California).