0
New Instrument |

Operating in the Dark:  A Night-Vision System for Surgery in Retinas Susceptible to Light Damage

András M. Komáromy, DrMedVet, PhD; Gregory M. Acland, BVSc; Gustavo D. Aguirre, VMD, PhD
Arch Ophthalmol. 2008;126(5):714-717. doi:10.1001/archopht.126.5.714.
Text Size: A A A
Published online

A standard operating microscope was modified with a bandpass infrared filter in the light path and infrared image intensifiers for each of the 2 eyepieces. We evaluated this system for subretinal injections in normal control dogs and those with a mutation in the rhodopsin gene. Rhodopsin-mutant dogs are a model for human autosomal dominant retinitis pigmentosa, and their retinas degenerate faster when exposed to modest light levels as used in routine clinical examinations. We showed that the mutant retinas developed severe generalized degeneration when exposed to the standard operating microscope light but not the infrared light. The modified operating microscope provided an excellent view of the ocular fundus under infrared illumination and allowed us to perform subretinal injections in the retinas of the rhodopsin-mutant dogs without any subsequent light-induced retinal degeneration.

Figures in this Article

Sign In to Access Full Content

Don't have Access?

Register and get free email Table of Contents alerts, saved searches, PowerPoint downloads, CME quizzes, and more

Subscribe for full-text access to content from 1998 forward and a host of useful features

Activate your current subscription (AMA members and current subscribers)

Purchase Online Access to this article for 24 hours

Figures

Place holder to copy figure label and caption
Figure 1.

The Zeiss OPMI 6 operating microscope (Carl Zeiss Inc, Oberkochen, Germany) modified with the monocular night-vision system (Owl Nitemare Third Generation; BE Meyers & Co, Inc, Redmond, Washington). The arrow points to the location of the slider housing the infrared bandpass filter.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 2.

Photomicrographs show retinas of rhodopsin-mutant dogs 15 days after subretinal injection of isotonic sodium chloride solution. With the use of the standard operating microscope light, the retinas showed extensive outer retinal and retina pigment epithelial degeneration in the injected (A and B) and noninjected (C and D) areas of different dogs. With the use of the night-vision system and infrared light in the fellow eyes, the retinal morphologic features remained normal in the injected areas (E and F) (hematoxylin-eosin; calibration marker, 50 μm).

Graphic Jump Location
Place holder to copy figure label and caption
Figure 3.

Comparison of brightness settings between the OPMI6 and VISU160 operating microscopes (Carl Zeiss Inc, Oberkochen, Germany). The gray area shows the range of commonly used VISU160 settings (0.3-0.6) that were overlapped with the OPMI6 settings evaluated in this study (3.5-5.0).

Graphic Jump Location

Tables

References

Correspondence

CME
Meets CME requirements for:
Browse CME for all U.S. States
Accreditation Information
The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity. Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
Commitment to Change (optional):
Indicate what change(s) you will implement in your practice, if any, based on this CME course.
Your quiz results:
The filled radio buttons indicate your responses. The preferred responses are highlighted
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.
NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).
Submit a Comment

Multimedia

Some tools below are only available to our subscribers or users with an online account.

Sign In to Access Full Content

Related Content

Customize your page view by dragging & repositioning the boxes below.

Articles Related By Topic
Related Topics
Jobs
brightcove.createExperiences();