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Research Letters |

Introduction of Epithelial Cells in the Flap-Graft Interface During Descemet Stripping Automated Endothelial Keratoplasty FREE

Isabelle E. Y. Saelens, MD; Marjolijn C. Bartels, MD, PhD; Gabriel Van Rij, MD, PhD; Winand N. M. Dinjens, PhD; Cornelia M. Mooy, MD, PhD
Arch Ophthalmol. 2009;127(7):936-945. doi:10.1001/archophthalmol.2009.143.
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Microkeratome-assisted Descemet stripping automated endothelial keratoplasty (DSAEK) is a form of lamellar corneal surgery that allows for the selective replacement of diseased endothelium.13 The DSAEK procedure is relatively new. So far, few complications have been described; these include graft detachment and graft failure.4 Recently, epithelial ingrowth in the flap-graft interface after DSAEK has been reported.5 We report a case of introduction of epithelial cells, originating from the donor tissue, in the flap-graft interface.

In May 2005, a 67-year-old woman visited with pseudophakic bullous keratopathy in the right eye. Best-corrected visual acuity was 0.08 OD. Because of the vision-limiting bullous keratopathy in the right eye, a DSAEK was performed under general anesthesia. A cornea from a male donor was used.

One week postoperatively, the posterior lamellar graft was detached inferiorly and nasally. An additional air bubble and two 10-0 nylon sutures were placed to fixate the transplant. Thereafter, partial adherence of the lamella was seen.

Four months postoperatively, the graft was attached and best-corrected visual acuity improved to 0.63 OD, with −1.00. Between the 3-o’clock and 6-o’clock positions, anterior synechia were present and the interface was slightly hazy. During regular follow-up, the abnormalities remained stable under fluorometholone, 1%, eyedrops. The intraocular pressure remained between 10 and 17 mm Hg during follow-up.

One year after DSAEK, the patient had progressive blurring of vision to hand movements. We observed total corneal decompensation with bullae and Descemet membrane folds. Therapy with prednisolone acetate, 10%, eyedrops (Pred Forte) 6 times a day, prednisolone pivalate, 5%, eye gel (Ultracortenol) 3 times a day, and preservative-free chloramphenicol, 0.4%, eyedrops 3 times a day proved to be unsuccessful. A penetrating posterior mushroom keratoplasty was performed. Six months after surgery, best-corrected visual acuity improved to 0.80 OD with –3.25 – 2.00 × 30°. The corneal graft in the right eye has remained clear.

Histopathologic examination of the removed corneal button demonstrated 2 cysts at the interface between the recipient cornea and the donor stroma. One cyst was lined by squamous epithelium; a smaller, centrally located cyst contained eosinophilic granular material (Figure 1A). The Descemet membrane with some excrescences was incarcerated in the surgical scar. There was bullous keratopathy of the corneal epithelium. On immunohistochemistry, the lining of the cyst as well as the granular material stained strongly positive for the epithelial markers 34Be12 and keratin 5/6, identical to surface epithelium (Figure 1B). The granular material was consistent with degenerated epithelial cells. In situ hybridization with X and Y probes revealed 2 X chromosomes in the nuclei of the epithelium and the stroma of the recipient. The nuclei of the epithelium of the cyst wall contained 1 X chromosome and 1 Y chromosome (Figure 2).

Place holder to copy figure label and caption
Figure 1.

There are 2 cysts at the interface between the recipient cornea and the posterior endothelial graft tissue (A), and the lining of the cysts stains strongly positive for the epithelial markers 34Be12 and keratin 5/6 (B).

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Place holder to copy figure label and caption
Figure 2.

In situ hybridization with X and Y chromosome probes revealed 2 X chromosomes (arrow) in the epithelium and the stroma of the recipient (A) and 1 X chromosome (arrow) in the epithelium of the cyst (B). C, The epithelium of the cyst wall contained 1 Y chromosome (arrows). D, There was no staining of the Y chromosome probe (arrow) in the tissue of the recipient.

Graphic Jump Location

In our patient, the DSAEK was complicated by introduction of epithelial cells along the interface. Her native Descemet membrane with Fuchs endothelium dystrophy was partially incarcerated in the surgical scar, similar to the earlier described case,5 suggesting an invasive postoperative ingrowth process. The X-Y karyotyping revealed that the epithelial cells were of donor origin. The technique of posterior lamellar keratoplasty is relatively new and technically difficult, with a surgeon's learning curve. The most critical step is the preparation of the posterior lamellar disc by hand or by use of a microkeratome. We postulate that the donor epithelium was implanted during the preparation of the donor posterior lamellar disc and was introduced intraoperatively. If complete attachment of the donor posterior lamella is accomplished, ectopic epithelial cells in the interface might remain stable without proliferation. In a partially detached donor lamella, ectopic epithelial cells might proliferate along the recipient's posterior cornea.6

Correspondence: Dr Saelens, Department of Ophthalmology, Erasmus Medical Center, PO Box 2040, 3000 CA Rotterdam, the Netherlands (i.saelens@erasmusmc.nl).

Financial Disclosure: None reported.

Price  FW  JrPrice  MO Descemet's stripping with endothelial keratoplasty in 200 eyes: early challenges and technique to enhance donor adherence. J Cataract Refract Surg 2006;32 (3) 411- 418
PubMed
Gorovoy  MS Descemet-stripping automated endothelial keratoplasty. Cornea 2006;25 (8) 886- 889
PubMed
Koenig  SBCovert  DJDupps  WJ  JrMeisler  DM Visual acuity, refractive error, and endothelial cell density six months after Descemet stripping and automated endothelial keratoplasty (DSAEK). Cornea 2007;26 (6) 670- 674
PubMed
Suh  LHYoo  SHDeobhakta  A  et al.  Complications of Descemet's stripping with automated endothelial keratoplasty: survey of 118 eyes at one institute. Ophthalmology 2008;115 (9) 1517- 1524
PubMed
Walker  BMHindman  HBEbrahimi  KB  et al.  Epithelial downgrowth following Descemet's-stripping automated endothelial keratoplasty. Arch Ophthalmol 2008;126 (2) 278- 280
PubMed
Davanger  MOlsen  EG Experimental epithelial ingrowth: epithelial/endothelial interaction through a corneal perforation, studied in organ culture. Acta Ophthalmol (Copenh) 1985;63 (4) 443- 448
PubMed

Figures

Place holder to copy figure label and caption
Figure 1.

There are 2 cysts at the interface between the recipient cornea and the posterior endothelial graft tissue (A), and the lining of the cysts stains strongly positive for the epithelial markers 34Be12 and keratin 5/6 (B).

Graphic Jump Location
Place holder to copy figure label and caption
Figure 2.

In situ hybridization with X and Y chromosome probes revealed 2 X chromosomes (arrow) in the epithelium and the stroma of the recipient (A) and 1 X chromosome (arrow) in the epithelium of the cyst (B). C, The epithelium of the cyst wall contained 1 Y chromosome (arrows). D, There was no staining of the Y chromosome probe (arrow) in the tissue of the recipient.

Graphic Jump Location

Tables

References

Price  FW  JrPrice  MO Descemet's stripping with endothelial keratoplasty in 200 eyes: early challenges and technique to enhance donor adherence. J Cataract Refract Surg 2006;32 (3) 411- 418
PubMed
Gorovoy  MS Descemet-stripping automated endothelial keratoplasty. Cornea 2006;25 (8) 886- 889
PubMed
Koenig  SBCovert  DJDupps  WJ  JrMeisler  DM Visual acuity, refractive error, and endothelial cell density six months after Descemet stripping and automated endothelial keratoplasty (DSAEK). Cornea 2007;26 (6) 670- 674
PubMed
Suh  LHYoo  SHDeobhakta  A  et al.  Complications of Descemet's stripping with automated endothelial keratoplasty: survey of 118 eyes at one institute. Ophthalmology 2008;115 (9) 1517- 1524
PubMed
Walker  BMHindman  HBEbrahimi  KB  et al.  Epithelial downgrowth following Descemet's-stripping automated endothelial keratoplasty. Arch Ophthalmol 2008;126 (2) 278- 280
PubMed
Davanger  MOlsen  EG Experimental epithelial ingrowth: epithelial/endothelial interaction through a corneal perforation, studied in organ culture. Acta Ophthalmol (Copenh) 1985;63 (4) 443- 448
PubMed

Correspondence

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