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Ophthalmic Molecular Genetics |

Clinical and Genetic Profile of Avellino Corneal Dystrophy in 2 Families From North India FREE

Preeti Paliwal, MSc; Jaya Gupta, DNB, FICO; Radhika Tandon, MD, DNB, FRCS(Edin), FRCOpth; Arundhati Sharma, PhD; Rasik B. Vajpayee, MS, FRCS(Edin), FRANZCO
[+] Author Affiliations

Author Affiliations: Laboratory of Cyto-Molecular Genetics, Department of Anatomy (Ms Paliwal and Dr Sharma), and Cornea and Refractive Surgery Services, Dr Rajendra Prasad Centre for Ophthalmic Sciences (Ms Gupta and Dr Tandon), All India Institute of Medical Sciences, New Delhi; and Centre for Eye Research Australia, University of Melbourne, Melbourne, Australia (Dr Vajpayee).


Arch Ophthalmol. 2009;127(10):1373-1376. doi:10.1001/archophthalmol.2009.262.
Text Size: A A A
Published online

Objective  To report Avellino corneal dystrophy and underlying R124H mutation in 2 families of Indian origin.

Methods  Peripheral blood was collected in EDTA for genomic DNA isolation from leukocytes of all affected and unaffected individuals. Amplification of transforming growth factor β-induced gene (TGFBI) using polymerase chain reaction followed by direct sequencing was carried out to determine the mutations underlying the disorder. A detailed clinical evaluation was undertaken to establish a genotype-phenotype correlation.

Results  R124H mutation resulting from a missense heterozygous substitution of G to A at nucleotide 418 of TGFBI was detected in all affected members of the 2 families. The affected individuals were clinically diagnosed as having granular corneal dystrophy. Histopathological examination was not done because no surgical intervention was undertaken.

Conclusions  To our knowledge, this is the first report of Avellino corneal dystrophy from India clinically diagnosed as granular corneal dystrophy, emphasizing that TGFBI screening is essential for the accurate diagnosis and classification of corneal dystrophies.

Clinical Relevance  Molecular genetics is a useful tool for accurate diagnosis and classification of corneal dystrophies. All autosomal dominant stromal dystrophies should be screened for underlying mutations in TGFBI because the clinical and phenotypic appearance is variable.

Figures in this Article

Avellino corneal dystrophy is characterized by the presence of gray-white discrete granular deposits in the subepithelial and anterior stromal corneal layers with or without discernible lattice lines in the stroma.1,2 These lattice lines develop in the second and third decade or even later and differ from the ones present in typical lattice corneal dystrophy in being larger, denser, whiter, and more spiculated.3 In the most advanced form of the disorder, stromal haze emerges.1 Histologically, these deposits stain with Masson trichrome and Congo red and are seen as discrete hyaline and fusiform deposits of amyloid in the corneal stroma.46

Explicit mutations in TGFBI are responsible for specific types of 5q31-linked corneal dystrophies, such as Groenouw type I (R555W), Avellino (R124H), Reis-Bücklers (R124L), Thiel-Behnke (R555Q), and lattice type I (R124C).7 The R124H mutation known to be associated with Avellino corneal dystrophy (ACD) (OMIM 121900) was initially described in families originating from the Italian province near Naples.8 Subsequently, with widespread availability of molecular diagnostic techniques, it has also been reported from Germany,3 Ireland,9 Europe,7 Japan,1012 France,13 South Korea,14,15 the United Kingdom,16 and Iran.17

Despite specific TGFBI mutations being associated with each of these corneal dystrophies, atypical and variable phenotypes1821 along with extensive intrafamilial and interfamilial variations22 are seen and genotype-phenotype correlation is not always possible.

We studied 5 affected individuals from 2 unrelated, nonconsanguineous Indian families who presented clinically with granular corneal dystrophy and hereby report their clinical features with genetic analysis.

The study had the approval of the institute research ethics committee and conformed to the tenets of the Declaration of Helsinki. Informed consent from all participants was obtained for slitlamp examination, in vivo white-light confocal microscopy (Confoscan 4; Nidek Technologies, Padova, Italy), and molecular genetic studies. Detailed family history was taken and the available family members were examined.

All members of both families were born in India and have their origin in the North Indian state of Haryana. They have no relatives of Italian origin, making them the first Indian families, to our knowledge, to be reported to have ACD. The pedigree charts of the 2 families are shown in Figure 1.

Place holder to copy figure label and caption
Figure 1.

Pedigrees of patients having R124H mutations in TGFBI, showing segregation of mutations, disease status of individuals, and individuals examined (asterisks) in the 2 families A and B. Arrows indicate probands.

Graphic Jump Location

Peripheral blood samples (5 mL) were collected in EDTA and genomic DNA was extracted from all the samples. The DNA was then subjected to polymerase chain reaction (PCR) amplification using a set of primer pairs, as described previously.7 The constituents and the conditions used for PCR reaction are described herein. The 25-μL reaction mixture contained genomic DNA (200 ng), primers (0.5pM each), magnesium chloride (1.5mM), deoxyribonucleotide triphosphate (0.2mM), 1× PCR buffer (containing 10mM TRIS–hydrochloric acid, pH 8.3; 50mM potassium chloride; and 0.1% gelatin), and Taq polymerase (0.5 U; Roche, Basel, Switzerland). The PCR machine was programmed with the following amplification conditions of 3 minutes of denaturation at 94°C followed by 35 cycles at 94°C for 1 minute, 58°C for 1 minute and 72°C for 1 minute, and final extension was given at 72°C for 5 minutes.

Amplified products were sequenced directly with BigDye Terminator Mix version 3.1 (Applied Biosystems, Foster City, California), according to the manufacturer's instructions, and then were analyzed on an ABI-3100 Genetic Analyzer (Applied Biosystems). Nucleotide sequences for the coding regions were compared with the nucleotide sequence of the published TGFBI human complementary DNA (GenBank NM_000358).

FAMILY A

Case II-1 (proband) was first examined at the Cornea and External Eye Disease Clinic at the age of 51 years when he presented with visual disturbance. The patient's best-corrected visual acuity on initial examination was 6/9 OU. Slitlamp biomicroscopy showed discrete disc and ribbonlike opacities in the anterior stroma of the right eye (Figure 2A and B) while the left eye (Figure 2E and F) was found to have predominantly ribbonlike discrete opacities in the anterior stroma. The condition was diagnosed as granular corneal dystrophy.

Place holder to copy figure label and caption
Figure 2.

Slitlamp photographs of proband (case II-1, family A) showing symmetrical ribbonlike opacities in the anterior corneal stroma (right eye, A and B, left eye, E and F). Confocal images (right eye, C and D, left eye, G and H) showing hyperreflective focal deposits with irregular edges in the basal epithelium (C and G). Normal keratocyte nuclei are seen in the stroma (D and H).

Graphic Jump Location

Case III-2 (proband's daughter) was referred to the Cornea and External Eye Disease Clinic at the age of 19 years when she complained of redness and watering. The patient's unaided visual acuity was 6/6 OU. Slitlamp biomicroscopy revealed discrete ring- and crumb-shaped opacities distributed in a radiating pattern in the anterior stroma of both eyes (Figure 3), suggestive of granular corneal dystrophy. In addition, she also had a few discrete, nonbranching, linear, gray-white opacities in the anterior stroma (Figure 3B and D). Examination of the unaffected family members revealed a disease-free cornea.

Place holder to copy figure label and caption
Figure 3.

Slitlamp photographs of the proband's daughter (case III-2, family A) showing axially distributed crumblike opacities in the right (A and B) and left (C and D) eye. Arrows indicate the few gray-white, raylike, linear deposits in the superficial stroma.

Graphic Jump Location
FAMILY B

The second family was also clinically diagnosed as having granular corneal dystrophy and consisted of a 53-year-oldproband (case II-1), her 32-year-old daughter (case III-1), and a 28-year-old son (case III-2). Slitlamp examination of the proband (case II-1) showed discrete rings, crumbs, and a few raylike, linear, gray-white opacities in the anterior axial corneal stroma of both eyes, with more prominent clinical features in the right eye (Figure 4). The daughter (case III-1) also had asymmetric involvement, the right eye (Figure 5A and B) being more prominently affected, with similar features as the mother but more pronounced. The son (case III-3) had a unilateral presentation with a single discrete opacity in the right eye (Figure 6A).

Place holder to copy figure label and caption
Figure 4.

Slitlamp photographs of the proband (case II-1, family B) showing a milder phenotype in the right (A and B) and left (C and D) eye. Arrows indicate faintly visible linear deposits.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 5.

Slitlamp photographs of the proband's daughter (case III-1, family B). The right eye is more prominently affected (A and B). The gray-white linear deposits are indicated by the arrows (B and D).

Graphic Jump Location
Place holder to copy figure label and caption
Figure 6.

Slitlamp photographs of the proband's son (case III-2, family B). An isolated gray-white lesion is seen in the right eye (A) (white arrow). Also seen is a persistent pupillary membrane (yellow arrow). The left eye appeared normal (B).

Graphic Jump Location

Direct sequencing analysis of exon 4 of TGFBI in the 2 affected members from family A and the 3 affected members of family B revealed a single heterozygous missense substitution at nucleotide position 418 (G to A) converting arginine at codon 124 to histidine (Figure 7). This mutation results in a change in the structure of the protein coded by TGFBI, resulting in hyaline and amyloid deposition in the corneal stroma, and is known to be associated with cases of ACD. Similar change at this position was not seen in the unaffected members of both the families.

Place holder to copy figure label and caption
Figure 7.

Chromatogram showing R124H mutations in TGFBI of patients.

Graphic Jump Location

The families with ACD initially reported in the literature were found to trace their ancestry to a large region of Campania in Italy comprising cities like Naples, Avellino, Lioni, and Stio.8 Many cases of ACD have subsequently been reported from other countries.3,7,917 This study describes for the first time, to our knowledge, ACD from India in 2 families with R124H mutation, thus emphasizing the geographic diversity of the disease.

Avellino corneal dystrophy is an autosomal dominant disorder, sharing its features with both granular and lattice corneal dystrophy, and results from a specific mutation (R124H) in TGFBI.7 In our study of the 5 affected individuals, 3 individuals (family A case III-2 and family B cases II-1 and III-1) showed the presence of a few fine, gray-white linear deposits in the superficial stroma. These deposits were different from those classically seen in lattice corneal dystrophy as these were not refractile, translucent, or branching in pattern. Two affected individuals in their sixth decade (family A case I-1 and family B case II-1) harbored the heterozygous R124H mutation but did not show any lattice lesions that are classically described with progression of age in ACD. Confocal microscopy of 3 affected individuals (family A case II-1 and family B cases II-1 and III-1) revealed highly reflective granular lesions in the superficial stroma of the central cornea with no other images corresponding to latticelike lesions.

Histopathologically, corneal dystrophies are classified based on the nature of the deposits seen in the cornea. Granular corneal dystrophy and its subtypes are marked by the presence of hyaline deposits while lattice corneal dystrophy, along with its variants, is characterized by the presence of amyloid deposits. Avellino corneal dystrophy is unique as it shows the presence of both hyaline and amyloid deposits. However, histopathological examination does not obviate the need for genetic analysis because it may be inconclusive and may not be available if surgical intervention is not undertaken. Classification of corneal dystrophies based only on clinical and histopathological characteristics is becoming increasingly difficult with the identification of new genotypic variants with different phenotypic features. This is evident from 1 such study23 that reports a new combined granular lattice dystrophy with atypical presentation that could not be classified based only on clinical and histopathological evidence. Molecular genetic analysis revealed a new causative mutation M619K in TGFBI in the family, without which the dystrophy would have been classified as a phenotypic variant of ACD and the new mutation would have been missed. It has also been reported that the heterozygous R124H mutation of TGFBI resulted in an atypical form of granular corneal dystrophy that was characterized histopathologically by the absence of amyloid deposits,16,24 thereby indicating that for the correct diagnosis of such dystrophies, histopathological and genetic evaluations are both essential. In our study, histopathological examination was not done because none of the affected individuals required any surgical intervention.

Our results confirm the importance of TGFBI screening as a mandatory step for the accurate diagnosis and classification of corneal dystrophies that would facilitate timely intervention and management, particularly in the younger age group.

Correspondence: Arundhati Sharma, PhD, Laboratory of Cyto-Molecular Genetics, Room 1015, Department of Anatomy, All India Institute of Medical Sciences, New Delhi De 110029, India (arundhatisharma1@gmail.com).

Submitted for Publication: March 31, 2009; final revision received May 4, 2009; accepted May 10, 2009.

Author Contributions: Mss Paliwal and Gupta contributed equally to this study.

Financial Disclosure: None reported.

Funding/Support: This work was supported by the Department of Biotechnology and the Council of Scientific and Industrial Research, Ministry of Science and Technology, Government of India.

Additional Contributions: Meena Verma, BSc, took the clinical photographs and confocal images. We thank the patients and their family members for participating in this study.

Holland  EJDaya  SMStone  EM  et al.  Avellino corneal dystrophy: clinical manifestations and natural history. Ophthalmology 1992;99 (10) 1564- 1568
PubMed Link to Article
Lucarelli  MJAdamis  AP Avellino corneal dystrophy. Arch Ophthalmol 1994;112 (3) 418- 419
PubMed Link to Article
Rosenwasser  GOSucheski  BMRosa  N  et al.  Phenotypic variation in combined granular-lattice (Avellino) corneal dystrophy. Arch Ophthalmol 1993;111 (11) 1546- 1552
PubMed Link to Article
Garner  A Histochemistry of corneal granular dystrophy. Br J Ophthalmol 1969;53 (12) 799- 807
PubMed Link to Article
Akiya  SBrown  SI Granular dystrophy of the cornea: characteristic electron microscopic lesion. Arch Ophthalmol 1970;84 (2) 179- 192
PubMed Link to Article
Owens  SLSugar  JEdward  DP Superficial granular corneal dystrophy with amyloid deposit. Arch Ophthalmol 1992;110 (2) 175- 176
PubMed Link to Article
Munier  FLKorvatska  EDjemai  A  et al.  Kerato-epithelin mutations in four 5q31 linked corneal dystrophies. Nat Genet 1997;15 (3) 247- 251
PubMed Link to Article
Folberg  RAlfonso  ECroxatto  JO  et al.  Clinically atypical granular corneal dystrophy with pathologic features of lattice-like amyloid deposits: a study of three families. Ophthalmology 1988;95 (1) 46- 51
PubMed Link to Article
Kennedy  SMO McNamara  MHillery  MHurley  CCollum  LMGiles  S Combined granular lattice dystrophy (Avellino corneal dystrophy). Br J Ophthalmol 1996;80 (5) 489- 490
PubMed Link to Article
Konishi  MMashima  YNakamura  YYamada  MSugiura  H Granular-lattice (Avellino) corneal dystrophy in Japanese patients. Cornea 1997;16 (6) 635- 638
PubMed Link to Article
Konishi  MYamada  MNakamura  YMashima  Y Varied appearance of cornea of patients with corneal dystrophy associated with R124H mutation in the BIGH3 gene. Cornea 1999;18 (4) 424- 429
PubMed Link to Article
Akimune  CWatanabe  HMaeda  N  et al.  Corneal guttata associated with the corneal dystrophy resulting from a Big-h3 R124H mutation. Br J Ophthalmol 2000;84 (1) 67- 71
PubMed Link to Article
Dighiero  PEllies  PValleix  S  et al.  Avellino dystrophy: current diagnostic criteria [in French]. J Fr Ophtalmol 2000;23 (7) 735- 737
PubMed
Kim  HSYoon  SKCho  BJKim  EKJoo  CK BIGH3 gene mutation and rapid detection in Korean patients with corneal dystrophy. Cornea 2001;20 (8) 844- 849
PubMed Link to Article
Jun  RMTchah  HKim  TI  et al.  Avellino corneal dystrophy after LASIK. Ophthalmology 2004;111 (3) 463- 468
PubMed Link to Article
El-Ashry  MFAbd El-Aziz  MMLarkin  DF  et al.  A clinical, histopathological, and genetic study of Avellino corneal dystrophy in British families. Br J Ophthalmol 2003;87 (7) 839- 842
PubMed Link to Article
Alavi  AElahi  ERahmati-Kamel  MKarimian  FRezaei-Kanavi  M Mutation screening of TGFBI in two Iranian Avellino corneal dystrophy pedigrees. Clin Experiment Ophthalmol 2008;36 (1) 26- 30
PubMed Link to Article
Aldave  AJRayner  SAKim  BTPrechanond  AYellore  VS Unilateral lattice corneal dystrophy associated with the novel His572del mutation in the TGFBI gene. Mol Vis 2006;12142- 146
PubMed
Aldave  AJRayner  SAKing  JAAffeldt  JAYellore  VS A unique corneal dystrophy of Bowman's layer and stroma associated with the Gly623Asp mutation in the transforming growth factor β-induced (TGFBI) gene. Ophthalmology 2005;112 (6) 1017- 1022
PubMed Link to Article
Aldave  AJGutmark  JGYellore  VS  et al.  Lattice corneal dystrophy associated with the Ala546Asp and Pro551Gln missense changes in the TGFBI gene. Am J Ophthalmol 2004;138 (5) 772- 781
PubMed Link to Article
Kannabiran  CKlintworth  GK TGFBI gene mutations in corneal dystrophies. Hum Mutat 2006;27 (7) 615- 625
PubMed Link to Article
Klintworth  GK Advances in the molecular genetics of corneal dystrophies. Am J Ophthalmol 1999;128 (6) 747- 754
PubMed Link to Article
Aldave  AJYellore  VSSonmez  B  et al.  A novel variant of combined granular-lattice corneal dystrophy associated with the Met619Lys mutation in the TGFBI gene. Arch Ophthalmol 2008;126 (3) 371- 377
PubMed Link to Article
Stewart  HSRidgway  AEDixon  MJBonshek  RParveen  RBlack  G Heterogeneity in granular corneal dystrophy: identification of three causative mutations in the TGFBI (BIGH3) gene-lessons for corneal amyloidogenesis. Hum Mutat 1999;14 (2) 126- 132
PubMed Link to Article

Figures

Place holder to copy figure label and caption
Figure 1.

Pedigrees of patients having R124H mutations in TGFBI, showing segregation of mutations, disease status of individuals, and individuals examined (asterisks) in the 2 families A and B. Arrows indicate probands.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 2.

Slitlamp photographs of proband (case II-1, family A) showing symmetrical ribbonlike opacities in the anterior corneal stroma (right eye, A and B, left eye, E and F). Confocal images (right eye, C and D, left eye, G and H) showing hyperreflective focal deposits with irregular edges in the basal epithelium (C and G). Normal keratocyte nuclei are seen in the stroma (D and H).

Graphic Jump Location
Place holder to copy figure label and caption
Figure 3.

Slitlamp photographs of the proband's daughter (case III-2, family A) showing axially distributed crumblike opacities in the right (A and B) and left (C and D) eye. Arrows indicate the few gray-white, raylike, linear deposits in the superficial stroma.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 4.

Slitlamp photographs of the proband (case II-1, family B) showing a milder phenotype in the right (A and B) and left (C and D) eye. Arrows indicate faintly visible linear deposits.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 5.

Slitlamp photographs of the proband's daughter (case III-1, family B). The right eye is more prominently affected (A and B). The gray-white linear deposits are indicated by the arrows (B and D).

Graphic Jump Location
Place holder to copy figure label and caption
Figure 6.

Slitlamp photographs of the proband's son (case III-2, family B). An isolated gray-white lesion is seen in the right eye (A) (white arrow). Also seen is a persistent pupillary membrane (yellow arrow). The left eye appeared normal (B).

Graphic Jump Location
Place holder to copy figure label and caption
Figure 7.

Chromatogram showing R124H mutations in TGFBI of patients.

Graphic Jump Location

Tables

References

Holland  EJDaya  SMStone  EM  et al.  Avellino corneal dystrophy: clinical manifestations and natural history. Ophthalmology 1992;99 (10) 1564- 1568
PubMed Link to Article
Lucarelli  MJAdamis  AP Avellino corneal dystrophy. Arch Ophthalmol 1994;112 (3) 418- 419
PubMed Link to Article
Rosenwasser  GOSucheski  BMRosa  N  et al.  Phenotypic variation in combined granular-lattice (Avellino) corneal dystrophy. Arch Ophthalmol 1993;111 (11) 1546- 1552
PubMed Link to Article
Garner  A Histochemistry of corneal granular dystrophy. Br J Ophthalmol 1969;53 (12) 799- 807
PubMed Link to Article
Akiya  SBrown  SI Granular dystrophy of the cornea: characteristic electron microscopic lesion. Arch Ophthalmol 1970;84 (2) 179- 192
PubMed Link to Article
Owens  SLSugar  JEdward  DP Superficial granular corneal dystrophy with amyloid deposit. Arch Ophthalmol 1992;110 (2) 175- 176
PubMed Link to Article
Munier  FLKorvatska  EDjemai  A  et al.  Kerato-epithelin mutations in four 5q31 linked corneal dystrophies. Nat Genet 1997;15 (3) 247- 251
PubMed Link to Article
Folberg  RAlfonso  ECroxatto  JO  et al.  Clinically atypical granular corneal dystrophy with pathologic features of lattice-like amyloid deposits: a study of three families. Ophthalmology 1988;95 (1) 46- 51
PubMed Link to Article
Kennedy  SMO McNamara  MHillery  MHurley  CCollum  LMGiles  S Combined granular lattice dystrophy (Avellino corneal dystrophy). Br J Ophthalmol 1996;80 (5) 489- 490
PubMed Link to Article
Konishi  MMashima  YNakamura  YYamada  MSugiura  H Granular-lattice (Avellino) corneal dystrophy in Japanese patients. Cornea 1997;16 (6) 635- 638
PubMed Link to Article
Konishi  MYamada  MNakamura  YMashima  Y Varied appearance of cornea of patients with corneal dystrophy associated with R124H mutation in the BIGH3 gene. Cornea 1999;18 (4) 424- 429
PubMed Link to Article
Akimune  CWatanabe  HMaeda  N  et al.  Corneal guttata associated with the corneal dystrophy resulting from a Big-h3 R124H mutation. Br J Ophthalmol 2000;84 (1) 67- 71
PubMed Link to Article
Dighiero  PEllies  PValleix  S  et al.  Avellino dystrophy: current diagnostic criteria [in French]. J Fr Ophtalmol 2000;23 (7) 735- 737
PubMed
Kim  HSYoon  SKCho  BJKim  EKJoo  CK BIGH3 gene mutation and rapid detection in Korean patients with corneal dystrophy. Cornea 2001;20 (8) 844- 849
PubMed Link to Article
Jun  RMTchah  HKim  TI  et al.  Avellino corneal dystrophy after LASIK. Ophthalmology 2004;111 (3) 463- 468
PubMed Link to Article
El-Ashry  MFAbd El-Aziz  MMLarkin  DF  et al.  A clinical, histopathological, and genetic study of Avellino corneal dystrophy in British families. Br J Ophthalmol 2003;87 (7) 839- 842
PubMed Link to Article
Alavi  AElahi  ERahmati-Kamel  MKarimian  FRezaei-Kanavi  M Mutation screening of TGFBI in two Iranian Avellino corneal dystrophy pedigrees. Clin Experiment Ophthalmol 2008;36 (1) 26- 30
PubMed Link to Article
Aldave  AJRayner  SAKim  BTPrechanond  AYellore  VS Unilateral lattice corneal dystrophy associated with the novel His572del mutation in the TGFBI gene. Mol Vis 2006;12142- 146
PubMed
Aldave  AJRayner  SAKing  JAAffeldt  JAYellore  VS A unique corneal dystrophy of Bowman's layer and stroma associated with the Gly623Asp mutation in the transforming growth factor β-induced (TGFBI) gene. Ophthalmology 2005;112 (6) 1017- 1022
PubMed Link to Article
Aldave  AJGutmark  JGYellore  VS  et al.  Lattice corneal dystrophy associated with the Ala546Asp and Pro551Gln missense changes in the TGFBI gene. Am J Ophthalmol 2004;138 (5) 772- 781
PubMed Link to Article
Kannabiran  CKlintworth  GK TGFBI gene mutations in corneal dystrophies. Hum Mutat 2006;27 (7) 615- 625
PubMed Link to Article
Klintworth  GK Advances in the molecular genetics of corneal dystrophies. Am J Ophthalmol 1999;128 (6) 747- 754
PubMed Link to Article
Aldave  AJYellore  VSSonmez  B  et al.  A novel variant of combined granular-lattice corneal dystrophy associated with the Met619Lys mutation in the TGFBI gene. Arch Ophthalmol 2008;126 (3) 371- 377
PubMed Link to Article
Stewart  HSRidgway  AEDixon  MJBonshek  RParveen  RBlack  G Heterogeneity in granular corneal dystrophy: identification of three causative mutations in the TGFBI (BIGH3) gene-lessons for corneal amyloidogenesis. Hum Mutat 1999;14 (2) 126- 132
PubMed Link to Article

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