After informed consent was obtained, blood samples were obtained at the 7-month and 41-month follow-up visits to test for melanoma-associated retinopathy (MAR) autoantibodies, other antiretinal autoantibodies, and anti-RPE autoantibodies. A serum sample from the 7-month follow-up was initially analyzed (using commercially obtained purified proteins) for the presence of antiretinal antibodies to the following: aldolase C, aldolase A, carbonic anhydrase II, recoverin, heat shock protein 1, S-arrestin, and alpha-enolase. Serum samples from both time points were subsequently tested for autoantibodies against human donor retinal extract and primary nonimmortalized human RPE cell culture extract using Western immunoblots. Briefly, 20 μg of total protein extract was loaded per lane for sodium dodecyl sulfate–polyacrylamide gel electrophoresis; separated proteins were transferred onto a nitrocellulose membrane. The blots were blocked in 3% milk–phosphate-buffered saline–Tween for 1 hour, incubated with the patient's serum at 1:100 dilution overnight, and then incubated with horseradish peroxidase–conjugated goat antihuman IgG (Chemicon, Temecula, California) at 1:5000 dilution for 90 minutes. Target protein bands were detected using a commercially available kit (SuperSignal West Pico Chemiluminescent Substrate; Thermo Fisher Scientific, Rockford, Illinois). Bands identified by Western immunoblots were further analyzed by 2-dimensional gel electrophoresis and Western immunoblots, followed by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) tandem mass spectrometry (MS/MS) analysis. Last, the RPE MS/MS results were confirmed by Western immunoblots with purified protein (Abcam, Cambridge, Massachusetts, and Abnova, Taiwan). Our institutional review board determined that review and oversight of this project were not required.