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ARTICLE |

Effect of Fibrin on Morphologic Characteristics of Retinal Pigment Epithelial Cells FREE

Jesus S. Vidaurri-Leal, MD; Bert M. Glaser, MD
[+] Author Affiliations

Accepted for publication March 29, 1984.

Reprint requests to The Wilmer Ophthalmological Institute, The Johns Hopkins Hospital, Baltimore, MD 21205 (Dr Glaser).

David G. Emmert and Dennis R. Cain provided photographic assistance.


Arch Ophthalmol. 1984;102(9):1376-1379. doi:10.1001/archopht.1984.01040031118037
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• Human retinal pigment epithelial (RPE) cells in culture, when overlaid by a fibrin clot, lose their normal epithelial morphologic features and migrate into the overlying clot as fibrocytelike cells. The behavior of human RPE cells on exposure to fibrin correlates well with the observed response of RPE in several ocular disorders in which fibrin deposition within the eye is an important feature. The mechanism of recognition and interaction between fibrin and RPE cells is unknown. The in vitro system used in our current studies allows the investigation of this interaction in a controlled environment. Further study of the interaction between human RPE cells and fibrin offers the possibility of improving our understanding and treatment of several blinding ocular disorders, including the disciform phase of senile macular degeneration, proliferative vitreoretinopathy, and the sequelae of ocular trauma.

REFERENCES

Hynes RO:  Fibronectin and its relation to cellular structure and behavior , in Hay ED (ed): Cell Biology of Extracellular Matrix . New York, Plenum Press, 1981;, pp 295-335.
Alberts B, Bray D, Lewis J, et al: Molecular Biology of the Cell . New York, Garland Publishing Inc, 1983;, pp 692-718.
Schepens CL: Retinal Detachment and Allied Diseases . Philadelphia, WB Saunders Co, 1983;, vol 2, pp 1147-1151.
Yanoff M, Fine BS: Ocular Pathology . Philadelphia, Harper & Row Publishers Inc, 1982;, p 522.
Gass JD:  Pathogenesis of disciform detachment of the neuroepithelium: I. General concepts and classification . Am J Ophthalmol 1967;;63:573-583.
Gass JD:  Pathogenesis of disciform detachment of the neuroepithelium: III. Senile disciform macular degeneration . Am J Ophthalmol 1967;;63:617-644.
Laqua H, Machemer R:  Clinical-pathological correlation in massive periretinal proliferation . Am J Ophthalmol 1975;;80:913-929.
Flood M, Gouras P, Kjeldbye H:  Growth characteristics and ultrastructure of human retinal pigment epithelium in vitro . Invest Ophthalmol Vis Sci 1980;;19:1309-1320.
Kramer RH, Gonzalez R, Nicolson GL:  Metastatic tumor cells adhere preferentially to the extracellular matrix underlying vascular endothelial cells . Int J Cancer 1980;;26:639-645.
Ali IV, Hynes RD:  Effects of LETS glycoprotein on cell motility . Cell 1978;;14:439-446.
Vidaurri-Leal J, Hohman R, Glaser BM:  Effect of vitreous on morphologic characteristics of retinal pigment epithelial cells: A new approach to the study of proliferative vitreoretinopathy . Arch Ophthalmol 1984;;102:1220-1223.
Cleary PE, Minckler DS, Ryan SJ:  Ultrastructure of traction retinal detachment in rhesus monkey eyes after a posterior penetrating injury . Am J Ophthalmol 1980;;90:829-845.

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Hynes RO:  Fibronectin and its relation to cellular structure and behavior , in Hay ED (ed): Cell Biology of Extracellular Matrix . New York, Plenum Press, 1981;, pp 295-335.
Alberts B, Bray D, Lewis J, et al: Molecular Biology of the Cell . New York, Garland Publishing Inc, 1983;, pp 692-718.
Schepens CL: Retinal Detachment and Allied Diseases . Philadelphia, WB Saunders Co, 1983;, vol 2, pp 1147-1151.
Yanoff M, Fine BS: Ocular Pathology . Philadelphia, Harper & Row Publishers Inc, 1982;, p 522.
Gass JD:  Pathogenesis of disciform detachment of the neuroepithelium: I. General concepts and classification . Am J Ophthalmol 1967;;63:573-583.
Gass JD:  Pathogenesis of disciform detachment of the neuroepithelium: III. Senile disciform macular degeneration . Am J Ophthalmol 1967;;63:617-644.
Laqua H, Machemer R:  Clinical-pathological correlation in massive periretinal proliferation . Am J Ophthalmol 1975;;80:913-929.
Flood M, Gouras P, Kjeldbye H:  Growth characteristics and ultrastructure of human retinal pigment epithelium in vitro . Invest Ophthalmol Vis Sci 1980;;19:1309-1320.
Kramer RH, Gonzalez R, Nicolson GL:  Metastatic tumor cells adhere preferentially to the extracellular matrix underlying vascular endothelial cells . Int J Cancer 1980;;26:639-645.
Ali IV, Hynes RD:  Effects of LETS glycoprotein on cell motility . Cell 1978;;14:439-446.
Vidaurri-Leal J, Hohman R, Glaser BM:  Effect of vitreous on morphologic characteristics of retinal pigment epithelial cells: A new approach to the study of proliferative vitreoretinopathy . Arch Ophthalmol 1984;;102:1220-1223.
Cleary PE, Minckler DS, Ryan SJ:  Ultrastructure of traction retinal detachment in rhesus monkey eyes after a posterior penetrating injury . Am J Ophthalmol 1980;;90:829-845.

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