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Punctate Keratitis Induced by Subconjunctivally Injected Microfilariae of Onchocerca lienalis FREE

Atef A. Sakla, DVM, PhD; John J. Donnelly, PhD; James B. Lok, PhD; Mahin Khatami, PhD; John H. Rockey, MD, PhD
[+] Author Affiliations

Accepted for publication Jan 10, 1986.

Reprint requests to Department of Ophthalmology, Scheie Eye Institute, 51 N 39th St, Philadelphia, PA 19104 (Dr Donnelly).


Arch Ophthalmol. 1986;104(6):894-898. doi:10.1001/archopht.1986.01050180128044
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• An experimental model of human onchocercal keratitis was produced by injecting guinea pigs subconjunctivally with microfilariae (Mf) of Onchocerca lienalis. Actively motile O lienalis Mf spontaneously penetrated the central cornea and produced gray-white midstromal opacities, 0.2 to 0.4 mm in diameter, resembling those of human onchocercal punctate keratitis. Histologically, small foci of eosinophil and mononuclear cell infiltration associated with small pockets of interstitial edema were present in the central corneal stroma, with eosinophil infiltrates in the conjunctiva, episclera, limbus, and ciliary body. The severity of the punctate keratitis was increased by repeated subconjunctival inoculations of Mf. Punctate lesions were not seen following subconjunctival injection of Mf in animals previously hyperimmunized by three subcutaneous injections of Mf. Hyperimmunization may produce an immune response capable of destroying the subconjunctivally injected Mf and preventing their migration into the central cornea.

REFERENCES

Taylor HR:  Onchocerciasis , in Duane TD (ed): Clinical Ophthalmology . New York, Harper & Row Publishers Inc, 1984;, vol 5, pp 1-12.
Ottesen EA:  Immunological aspects of lymphatic filariasis and onchocerciasis in man . Trans R Soc Trop Med Hyg 1984;;78( (suppl) ):9-18
Duke BOL, Anderson J:  A comparison of the lesions produced in the cornea of the rabbit eye by microfilariae of the forest and Sudan-savanna strains of Onchocerca volvulus from Cameroon: I. The clinical picture . Tropenmed Parasitol 1972;; 23:354-368.
Garner A, Duke BOL, Anderson J:  A comparison of the lesions produced in the cornea of the rabbit eye by microfilariae of the forest and Sudan-savanna strains of Onchocerca volvulus from Cameroon: II. The pathology . Tropenmed Parasitol 1973;;24:385-396.
Donnelly JJ, Rockey JH, Bianco AE, et al:  Aqueous humor and serum IgE antibody in experimental ocular Onchocerca infections of guinea pigs . Ophthalmic Res 1983;;15:61-67.
Donnelly JJ, Rockey JH, Bianco AE, et al:  Ocular immunopathologic findings of experimental onchocerciasis . Arch Ophthalmol 1984;; 102:628-634.
Bianco AE, Ham P, El Sinnary K, et al:  Large-scale recovery of Onchocerca microfilariae from naturally infected cattle and horses . Trans R Soc Trop Med Hyg 1980;;74:109-110.
Rodger FC:  The pathogenesis and pathology of ocular onchocerciasis: IV. The pathology . Am J Ophthalmol 1960;;49:560-590.
Townson S, Bianco AE:  Immunization of calves against the microfilariae of Onchocerca lienalis . J Helminthol 1982;;56:297-303.
Townson S, Bianco AE, Doenhoff MJ, et al:  Immunity to Onchocerca lienalis in mice: I. Resistance induced by the homologous parasite . Tropenmed Parasitol 1984;;35:202-208.

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Taylor HR:  Onchocerciasis , in Duane TD (ed): Clinical Ophthalmology . New York, Harper & Row Publishers Inc, 1984;, vol 5, pp 1-12.
Ottesen EA:  Immunological aspects of lymphatic filariasis and onchocerciasis in man . Trans R Soc Trop Med Hyg 1984;;78( (suppl) ):9-18
Duke BOL, Anderson J:  A comparison of the lesions produced in the cornea of the rabbit eye by microfilariae of the forest and Sudan-savanna strains of Onchocerca volvulus from Cameroon: I. The clinical picture . Tropenmed Parasitol 1972;; 23:354-368.
Garner A, Duke BOL, Anderson J:  A comparison of the lesions produced in the cornea of the rabbit eye by microfilariae of the forest and Sudan-savanna strains of Onchocerca volvulus from Cameroon: II. The pathology . Tropenmed Parasitol 1973;;24:385-396.
Donnelly JJ, Rockey JH, Bianco AE, et al:  Aqueous humor and serum IgE antibody in experimental ocular Onchocerca infections of guinea pigs . Ophthalmic Res 1983;;15:61-67.
Donnelly JJ, Rockey JH, Bianco AE, et al:  Ocular immunopathologic findings of experimental onchocerciasis . Arch Ophthalmol 1984;; 102:628-634.
Bianco AE, Ham P, El Sinnary K, et al:  Large-scale recovery of Onchocerca microfilariae from naturally infected cattle and horses . Trans R Soc Trop Med Hyg 1980;;74:109-110.
Rodger FC:  The pathogenesis and pathology of ocular onchocerciasis: IV. The pathology . Am J Ophthalmol 1960;;49:560-590.
Townson S, Bianco AE:  Immunization of calves against the microfilariae of Onchocerca lienalis . J Helminthol 1982;;56:297-303.
Townson S, Bianco AE, Doenhoff MJ, et al:  Immunity to Onchocerca lienalis in mice: I. Resistance induced by the homologous parasite . Tropenmed Parasitol 1984;;35:202-208.

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