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ARTICLE |

The Role of Breakdown of the Blood-Retinal Barrier in Cell-Injection Models of Proliferative Vitreoretinopathy FREE

Harsha A. Sen, MD; Thomas J. Robertson; Brian P. Conway, MD; Peter A. Campochiaro, MD
[+] Author Affiliations

Accepted for publication May 16, 1988.

Reprint requests to Department of Ophthalmology, University of Virginia Medical Center, Box 475, Charlottesville, VA 22908 (Dr Campochiaro).


Arch Ophthalmol. 1988;106(9):1291-1294. doi:10.1001/archopht.1988.01060140451051
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• Rabbits were given an intravitreous injection of 5.0 × 105 rabbit retinal pigment epithelial (RPE) cells, human RPE cells, or human dermal fibroblasts in one eye and an injection of vehicle alone in the other eye. Some rabbits were treated with retinal cryopexy or intravenous sodium iodate on the day before injection. Vitreous fluorophotometry (VFP) and fundus examinations were performed before and at various times after cell injections. Retinal detachments were graded by premortem ophthalmoscopic examinations and postmortem gross pathologic examinations. Eyes injected with cells had higher VFP readings than eyes injected with vehicle at all time points. Eyes injected with fibroblasts or rabbit RPE had significantly higher mean VFP values before the onset of retinal detachment than those injected with human RPE cells. Within each group, high levels of fluorescein leakage in the first week correlated well with severity of subsequent traction retinal detachment and the fibroblast and rabbit RPE groups had more severe detachments than the human RPE group. Treatment with cryopexy or sodium iodate resulted in higher VFP readings, a higher frequency of retinal detachments, and detachments that occurred earlier and that were more severe. These data demonstrate that intravitreous cells cause blood-retinal barrier breakdown in rabbits and that the amount and duration of this breakdown are important variables in retinal detachment formation.

REFERENCES

Rachal WS, Burton TC:  Changing concepts of failures after retinal detachment surgery . Arch Ophthalmol 1979;;97:480-483.
Machemer R, Laqua H:  Pigment epithelial proliferation in retinal detachment: Massive periretinal proliferation . Am J Ophthalmol 1975;;80:1-23.
Machemer R, van Horn D, Aaberg TM:  Pigment epithelial proliferation in human retinal detachment with massive periretinal proliferation . Am J Ophthalmol 1978;;85:181-191.
Campochiaro PA, Bryan JA, Conway BP, et al:  Intravitreal chemotactic and mitogenic activity: Implication of blood-retinal barrier breakdown . Arch Ophthalmol 1986;;104:1685-1687.
Campochiaro PA, Gaskin HC, Vinores SA:  Retinal cryopexy stimulates traction retinal detachment formation in the presence of an ocular wound . Arch Ophthalmol 1987;;105:1567-1570.
Campochiaro PA, Jerdan JA, Glaser BM:  Serum contains chemoattractants for human retinal pigment epithelial cells . Arch Ophthalmol 1984;;102:1830-1833.
Conway BP:  Technical variables in vitreous fluorophotometry . Graefes Arch Clin Exp Ophthalmol 1985;;222:194-201.
Fastenberg DM, Diddie KR, Sorgente N, et al:  A comparison of different cellular inocula in an experimental model of massive periretinal proliferation . Am J Ophthalmol 1982;;93:559-564.
Campochiaro PA, Jerdan JA, Glaser BM, et al:  Vitreous aspirates from patients with proliferative vitreoretinopathy stimulate retinal pigment epithelial cell migration . Arch Ophthalmol 1985;;103:1403-1405.
Fastenberg DM, Diddie KR, Dorey K, et al:  The role of cellular proliferation in an animal model of massive periretinal proliferation . Am J Ophthalmol 1982;;93:565-572.
Bryan JA, Campochiaro PA:  A retinal pigment epithelial cell-derived growth factor(s) . Arch Ophthalmol 1986;;104:422-425.
Byer NE:  Spontaneous disappearance of early postoperative preretinal retraction: A sequel of retinal detachment surgery . Arch Ophthalmol 1973;;90:133-135.
Cangemi FE, Pitta CG, Schwartz PL:  Spontaneous resolution of massive periretinal proliferation . Am J Ophthalmol 1982;;93:92-95.
De Juan E, Machemer R:  Spontaneous reattachment of the retina despite proliferative vitreoretinopathy . Am J Ophthalmol 1984;;97:428-433.

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Rachal WS, Burton TC:  Changing concepts of failures after retinal detachment surgery . Arch Ophthalmol 1979;;97:480-483.
Machemer R, Laqua H:  Pigment epithelial proliferation in retinal detachment: Massive periretinal proliferation . Am J Ophthalmol 1975;;80:1-23.
Machemer R, van Horn D, Aaberg TM:  Pigment epithelial proliferation in human retinal detachment with massive periretinal proliferation . Am J Ophthalmol 1978;;85:181-191.
Campochiaro PA, Bryan JA, Conway BP, et al:  Intravitreal chemotactic and mitogenic activity: Implication of blood-retinal barrier breakdown . Arch Ophthalmol 1986;;104:1685-1687.
Campochiaro PA, Gaskin HC, Vinores SA:  Retinal cryopexy stimulates traction retinal detachment formation in the presence of an ocular wound . Arch Ophthalmol 1987;;105:1567-1570.
Campochiaro PA, Jerdan JA, Glaser BM:  Serum contains chemoattractants for human retinal pigment epithelial cells . Arch Ophthalmol 1984;;102:1830-1833.
Conway BP:  Technical variables in vitreous fluorophotometry . Graefes Arch Clin Exp Ophthalmol 1985;;222:194-201.
Fastenberg DM, Diddie KR, Sorgente N, et al:  A comparison of different cellular inocula in an experimental model of massive periretinal proliferation . Am J Ophthalmol 1982;;93:559-564.
Campochiaro PA, Jerdan JA, Glaser BM, et al:  Vitreous aspirates from patients with proliferative vitreoretinopathy stimulate retinal pigment epithelial cell migration . Arch Ophthalmol 1985;;103:1403-1405.
Fastenberg DM, Diddie KR, Dorey K, et al:  The role of cellular proliferation in an animal model of massive periretinal proliferation . Am J Ophthalmol 1982;;93:565-572.
Bryan JA, Campochiaro PA:  A retinal pigment epithelial cell-derived growth factor(s) . Arch Ophthalmol 1986;;104:422-425.
Byer NE:  Spontaneous disappearance of early postoperative preretinal retraction: A sequel of retinal detachment surgery . Arch Ophthalmol 1973;;90:133-135.
Cangemi FE, Pitta CG, Schwartz PL:  Spontaneous resolution of massive periretinal proliferation . Am J Ophthalmol 1982;;93:92-95.
De Juan E, Machemer R:  Spontaneous reattachment of the retina despite proliferative vitreoretinopathy . Am J Ophthalmol 1984;;97:428-433.

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