0
January 1989, Vol 107, No. 1 >
ARTICLE | January 1989

Ultrastructural Location of Extracellular Matrix Components in the Optic Nerve Head FREE

Harry A. Quigley, MD; Janice A. Jerdan, PhD; Nancy L. L'Hernault, MA; John C. Morrison, MD
[+] Author Affiliations

Accepted for publication April 14, 1988.

Presented in part at the Annual Meeting of the Association for Research in Vision and Ophthalmology, Sarasota, Fla, May 5, 1988.

Reprint requests to Maumenee B-110, The Wilmer Institute, The Johns Hopkins Hospital, Baltimore, MD 21205 (Dr Quigley).


Arch Ophthalmol. 1989;107(1):123-129. doi:10.1001/archopht.1989.01070010125040
Text Size: A A A
Published online
Article
References
Comments

• The distributions of laminin and collagen types I through V were determined in optic nerve heads of cynomolgus monkeys using colloidal gold immunoelectron microscopy. Collagen types I and III localized to striated collagen fibrils in arterial walls throughout the optic nerve head and to fibrils comprising the core of the lamina cribrosa beams and optic nerve septa. Collagen type IV and laminin localized to basement membranes of all blood vessels and to basement membranes of astrocytes that lie between the laminar beams and line the optic nerve septa and pia mater. Collagen type V was detected both in striated collagen fibrils and in vascular and astrocyte basement membranes. These results provide a detailed understanding of the optic nerve head extracellular matrix distribution and illustrate a sensitive method for future study of its role in glaucomatous optic nerve damage.

REFERENCES

1 +
Quigley HA, Green WR:  The histology of human glaucoma cupping and optic nerve damage: Clinicopathologic correlation in 21 eyes . Ophthalmology 1979;;86:1803-1827.
2 +
Quigley HA, Addicks EM:  Chronic experimental glaucoma in primates: II. Effect of extended intraocular pressure elevation on optic nerve head and axonal transport . Invest Ophthalmol Vis Sci 1980;;19:137-152.
3 +
Minckler DS, Bunt AH, Klock IB:  Radioautographic and cytochemical ultrastructural studies of axoplasmic transport in the monkey optic nerve head . Invest Ophthalmol Vis Sci 1978;;17:33-50.
4 +
Quigley HA, Addicks EM, Green WR, et al:  Optic nerve damage in human glaucoma: II. The site of injury and susceptibility to damage . Arch Ophthalmol 1981;;99:635-649.
5 +
Anderson DR, Hendrickson A:  Effect of intraocular pressure on rapid axoplasmic transport in monkey optic nerve . Invest Ophthalmol Vis Sci 1974;;13:771-783.
6 +
Anderson DR:  Ultrastructure of human and monkey lamina cribrosa and optic nerve head . Arch Ophthalmol 1969;;82:800-814.
7 +
Hogan MJ, Alvarado JA, Weddell JE:  The optic nerve , in Histology of the Human Eye . Philadelphia, WB Saunders Co, 1971;, pp 561-571.
8 +
Timpl R, Dziadek M:  Structure, development, and molecular pathology of basement membranes . Int Rev Exp Pathol 1986;;29:1-112.
9 +
Farquhar MG:  The glomerular basement membrane: A selective macromolecular filter , in Hay ED (ed): Cell Biology of Extracellular Matrix . New York, Plenum Press, 1981;, pp 335-378.
10 +
Martinez-Hernandez A, Amenta PS:  The basement membrane in pathology . Lab Invest 1983;;48:656-677.
11 +
Drance SA:  Low-tension glaucoma: Enigma and opportunity . Arch Ophthalmol 1985;;103:1131-1133.
12 +
Levine RZ:  Low tension glaucoma: A critical review and new material . Surv Ophthalmol 1980;;24:621-663.
13 +
Tengroth B, Rehnberg M, Amitzboll:  A comparative analysis of the collagen type and distribution in the trabecular meshwork, sclera, lamina cribrosa and the optic nerve in the human eye . Acta Ophthalmol 1985;;63( (suppl) ):91-93.
14 +
Jeng S, Goldbaum M, Logemann RB, et al:  Extracellular matrix of the optic nerve . Invest Ophthalmol Vis Sci 1987;;28( (suppl) ):61.
15 +
Hernandez MR, Igoe F, Neufeld AH:  Extracellular matrix of the human optic nerve head . Am J Ophthalmol 1986;;102:139-148.
16 +
Hernandez MR, Luo XX, Igoe F, et al:  Extracellular matrix of the human lamina cribrosa . Am J Ophthalmol 1987;;104:567-576.
17 +
Morrison J, L'Hernault N, Jerdan J, et al: Extracellular matrix composition of the monkey optic nerve head. Invest Ophthalmol Vis Sci, in press.
18 +
Konomi H, Hayashi T, Sano J, et al:  Immunohistochemical localization of type I, III, and IV collagens in the sclera and choroid of bovine, rat, and normal and pathological human eyes . Biomed Res 1983;;4:451-458.
19 +
Quigley HA, Hohman RM:  Laser energy levels for trabecular meshwork damage in the primate eye . Invest Ophthalmol Vis Sci 1983;;24:1305-1307.
20 +
Quigley HA, Sanchez RM, Dunkelberger GR:  Chronic glaucoma selectively damages large optic nerve fibers . Invest Ophthalmol Vis Sci 1987;;28:913-920.
21 +
Altman LG, Schneider BG, Papermaster DS:  Rapid embedding of tissues in lowicryl K4M for immunoelectron microscopy . J Histochem Cytochem 1984;;32:1217-1223.
22 +
Erickson PA, Anderson DH, Fisher SK:  Use of uranyl acetate en bloc to improve tissue preservation and labeling for post-embedding immunoelectron microscopy . J Electron Microsc Tech 1987;;15:303-314.
23 +
McGarvey ML, Baron-Van Evercooren A, Kleinman HK, et al:  Synthesis and effects of basement membrane components in cultured rat Schwann cells . Dev Biol 1984;;105:18-28.
24 +
Quigley HA:  The pathogenesis of reversible cupping in congenital glaucoma . Am J Ophthalmol 1977;;84:358-370.
25 +
Birk DE, Fitch JM, Linsenmayer TF:  Organization of collagen types I and V in the embryonic chicken cornea . Invest Ophthalmol Vis Sci 1986;;27:1470-1477.
26 +
Konomi F, Hayashi T, Nakayasu K, et al:  Localization of type V collagen and type IV collagen in human cornea, lung, and skin . Am J Pathol 1984;;116:417-426.
27 +
Modesti A, Kalebic T, Scarpa S, et al:  Type V collagen in human amnion is a 12 nm fibrillar component of the pericellular interstitium . Eur J Cell Biol 1984;;35:246-255.
28 +
Smith LT, Holbrook KA, Madri JA:  Collagen types I, III, and V in human embryonic and fetal skin . Am J Anat 1986;;175:507-521.
29 +
Roll FJ, Madri JA, Albert J, et al:  Codistribution of collagen types IV and AB2 in basement membranes and mesangium of the kidney: An immunoferritin study of ultrathin frozen sections . J Cell Biol 1980;;85:597-616.
30 +
Murphy CG, Yun AJ, Newsome DA:  Localization of extracellular proteins of the human trabecular meshwork by indirect immunofluorescence . Am J Ophthalmol 1987;;104:33-43.
31 +
Konomi H, Hayashi T, Sano J, et al:  Immunohistochemical localization of type I, III, and IV collagens in the sclera and choroid of bovine, rat, and normal and pathological human eyes . Biomed Res 1983;;4:451-458.
32 +
Bailey AJ:  Structure, function and ageing of the collagens of the eye . Eye 1987;;1:175-183.
33 +
Schmut O:  The identification of type III collagen in calf and bovine cornea and sclera . Exp Eye Res 1977;;25:505-509.
34 +
Kelley FW, Morin JD, Vesely S:  Characterization of collagen from normal human sclera . Exp Eye Res 1984;;39:533-542.
35 +
Laurie GW, LeBlond CP, Martin GR:  Localization of type IV collagen, laminin, heparan sulfate proteoglycan, and fibronectin to the basal lamina of basement membranes . J Cell Biol 1982;;95:340-344.

First Page Preview

First page PDF preview

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

1 +
Quigley HA, Green WR:  The histology of human glaucoma cupping and optic nerve damage: Clinicopathologic correlation in 21 eyes . Ophthalmology 1979;;86:1803-1827.
2 +
Quigley HA, Addicks EM:  Chronic experimental glaucoma in primates: II. Effect of extended intraocular pressure elevation on optic nerve head and axonal transport . Invest Ophthalmol Vis Sci 1980;;19:137-152.
3 +
Minckler DS, Bunt AH, Klock IB:  Radioautographic and cytochemical ultrastructural studies of axoplasmic transport in the monkey optic nerve head . Invest Ophthalmol Vis Sci 1978;;17:33-50.
4 +
Quigley HA, Addicks EM, Green WR, et al:  Optic nerve damage in human glaucoma: II. The site of injury and susceptibility to damage . Arch Ophthalmol 1981;;99:635-649.
5 +
Anderson DR, Hendrickson A:  Effect of intraocular pressure on rapid axoplasmic transport in monkey optic nerve . Invest Ophthalmol Vis Sci 1974;;13:771-783.
6 +
Anderson DR:  Ultrastructure of human and monkey lamina cribrosa and optic nerve head . Arch Ophthalmol 1969;;82:800-814.
7 +
Hogan MJ, Alvarado JA, Weddell JE:  The optic nerve , in Histology of the Human Eye . Philadelphia, WB Saunders Co, 1971;, pp 561-571.
8 +
Timpl R, Dziadek M:  Structure, development, and molecular pathology of basement membranes . Int Rev Exp Pathol 1986;;29:1-112.
9 +
Farquhar MG:  The glomerular basement membrane: A selective macromolecular filter , in Hay ED (ed): Cell Biology of Extracellular Matrix . New York, Plenum Press, 1981;, pp 335-378.
10 +
Martinez-Hernandez A, Amenta PS:  The basement membrane in pathology . Lab Invest 1983;;48:656-677.
11 +
Drance SA:  Low-tension glaucoma: Enigma and opportunity . Arch Ophthalmol 1985;;103:1131-1133.
12 +
Levine RZ:  Low tension glaucoma: A critical review and new material . Surv Ophthalmol 1980;;24:621-663.
13 +
Tengroth B, Rehnberg M, Amitzboll:  A comparative analysis of the collagen type and distribution in the trabecular meshwork, sclera, lamina cribrosa and the optic nerve in the human eye . Acta Ophthalmol 1985;;63( (suppl) ):91-93.
14 +
Jeng S, Goldbaum M, Logemann RB, et al:  Extracellular matrix of the optic nerve . Invest Ophthalmol Vis Sci 1987;;28( (suppl) ):61.
15 +
Hernandez MR, Igoe F, Neufeld AH:  Extracellular matrix of the human optic nerve head . Am J Ophthalmol 1986;;102:139-148.
16 +
Hernandez MR, Luo XX, Igoe F, et al:  Extracellular matrix of the human lamina cribrosa . Am J Ophthalmol 1987;;104:567-576.
17 +
Morrison J, L'Hernault N, Jerdan J, et al: Extracellular matrix composition of the monkey optic nerve head. Invest Ophthalmol Vis Sci, in press.
18 +
Konomi H, Hayashi T, Sano J, et al:  Immunohistochemical localization of type I, III, and IV collagens in the sclera and choroid of bovine, rat, and normal and pathological human eyes . Biomed Res 1983;;4:451-458.
19 +
Quigley HA, Hohman RM:  Laser energy levels for trabecular meshwork damage in the primate eye . Invest Ophthalmol Vis Sci 1983;;24:1305-1307.
20 +
Quigley HA, Sanchez RM, Dunkelberger GR:  Chronic glaucoma selectively damages large optic nerve fibers . Invest Ophthalmol Vis Sci 1987;;28:913-920.
21 +
Altman LG, Schneider BG, Papermaster DS:  Rapid embedding of tissues in lowicryl K4M for immunoelectron microscopy . J Histochem Cytochem 1984;;32:1217-1223.
22 +
Erickson PA, Anderson DH, Fisher SK:  Use of uranyl acetate en bloc to improve tissue preservation and labeling for post-embedding immunoelectron microscopy . J Electron Microsc Tech 1987;;15:303-314.
23 +
McGarvey ML, Baron-Van Evercooren A, Kleinman HK, et al:  Synthesis and effects of basement membrane components in cultured rat Schwann cells . Dev Biol 1984;;105:18-28.
24 +
Quigley HA:  The pathogenesis of reversible cupping in congenital glaucoma . Am J Ophthalmol 1977;;84:358-370.
25 +
Birk DE, Fitch JM, Linsenmayer TF:  Organization of collagen types I and V in the embryonic chicken cornea . Invest Ophthalmol Vis Sci 1986;;27:1470-1477.
26 +
Konomi F, Hayashi T, Nakayasu K, et al:  Localization of type V collagen and type IV collagen in human cornea, lung, and skin . Am J Pathol 1984;;116:417-426.
27 +
Modesti A, Kalebic T, Scarpa S, et al:  Type V collagen in human amnion is a 12 nm fibrillar component of the pericellular interstitium . Eur J Cell Biol 1984;;35:246-255.
28 +
Smith LT, Holbrook KA, Madri JA:  Collagen types I, III, and V in human embryonic and fetal skin . Am J Anat 1986;;175:507-521.
29 +
Roll FJ, Madri JA, Albert J, et al:  Codistribution of collagen types IV and AB2 in basement membranes and mesangium of the kidney: An immunoferritin study of ultrathin frozen sections . J Cell Biol 1980;;85:597-616.
30 +
Murphy CG, Yun AJ, Newsome DA:  Localization of extracellular proteins of the human trabecular meshwork by indirect immunofluorescence . Am J Ophthalmol 1987;;104:33-43.
31 +
Konomi H, Hayashi T, Sano J, et al:  Immunohistochemical localization of type I, III, and IV collagens in the sclera and choroid of bovine, rat, and normal and pathological human eyes . Biomed Res 1983;;4:451-458.
32 +
Bailey AJ:  Structure, function and ageing of the collagens of the eye . Eye 1987;;1:175-183.
33 +
Schmut O:  The identification of type III collagen in calf and bovine cornea and sclera . Exp Eye Res 1977;;25:505-509.
34 +
Kelley FW, Morin JD, Vesely S:  Characterization of collagen from normal human sclera . Exp Eye Res 1984;;39:533-542.
35 +
Laurie GW, LeBlond CP, Martin GR:  Localization of type IV collagen, laminin, heparan sulfate proteoglycan, and fibronectin to the basal lamina of basement membranes . J Cell Biol 1982;;95:340-344.

Correspondence

Submit a Letter
CME Course for:


You need to register in order to view this quiz.


To understand the clinical management of acute heart failure syndromes.
Accreditation Information The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians.
The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity.
Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
Note: You must get at least of the answers correct to pass this quiz.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.
To view and print your certificate and access a summary of your CME courses go to My CME.
NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s “Cited By” API will populate this tab (http://www.crossref.org/citedby.html).
Compression-Only CPR: Pushing the Science Forward
Cone
AAM 2010;304:1493-1495.
All you need to read in the other general journals
BMJ 2010;341:c5893-c1494.
Submit a Comment

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

© 2012 American Medical Association. All Rights Reserved.
Powered bySilverchair Information Systems