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June 1990, Vol 108, No. 6 >
ARTICLE | June 1990

Suppression of Human Retinal Pigment Epithelial Cell Proliferation by Hyperthermia FREE

Teruyo Ohuchi, MD; Shoji Kuriyama, MD; Nagahisa Yoshimura, MD; Yoshihito Honda, MD; Masahiro Hiraoka, MD; Mitsuyuki Abe, MD
[+] Author Affiliations

Accepted for publication November 16, 1989.

Reprint requests to Department of Ophthalmology, Kyoto University Faculty of Medicine, Kyoto 606, Japan (Dr Yoshimura).


Arch Ophthalmol. 1990;108(6):873-875. doi:10.1001/archopht.1990.01070080117047
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• To investigate the effects of hyperthermia on the proliferation of retinal pigment epithelial cells, in vitro growth of cultured human retinal pigment epithelial cells was studied following heat treatment. Forty-eight hours after plating, heat treatment of 37°C and 41°C to 45°C for 30 or 60 minutes was given to retinal pigment epithelial cells. On days 1, 3, and 7, cell proliferation was evaluated by cell number counting and by DNA synthesis analysis. Hyperthermia gave statistically significant suppression of cell growth above 43°C heat treatment and absolute cell number reduction above 44°C on the seventh day. Deoxyribonucleic acid synthesis was significantly suppressed with 43°C for 60 minutes or above 44°C heat treatment. Hyperthermia may be a potential new therapy for proliferative vitreoretinopathy.

REFERENCES

1 +
Radtke ND, Tano Y, Chandler D, Machemer R.  Simulation of massive periretinal proliferation by autotransplantation of retinal pigment epithelial cells in rabbits . Am J Ophthalmol . 1981;;91:76-87.
2 +
Machemer R, Horn DV, Aaberg TM.  Pigment epithelial proliferation in human retinal detachment with massive periretinal proliferation . Am J Ophthalmol . 1978;;85:181-191.
3 +
Glaser BM, Cardin A, Biscoe B.  Proliferative vitreoretinopathy: the mechanism of development of vitreoretinal traction . Ophthalmology . 1987;;94:327-332.
4 +
Chandler DB, Rozakis G, de Juan E, Machemer R.  The effect of triamcinolone acetonide on a refined experimental model of proliferative vitreoretinopathy . Am J Ophthalmol . 1985;;99:686-690.
5 +
Blumenkranz M, Hernandez E, Ophir A, Norton EWD. 5-Fluorouracil:  new applications in complicated retinal detachment for an established antimetabolite . Ophthalmology . 1984;;91:122-130.
6 +
Stern WH, Lewis GP, Erickson PA, et al.  Fluorouracil therapy for proliferative vitreoretinopathy after vitrectomy . Am J Ophthalmol . 1983;;96:33-42.
7 +
Lemor M, de Bustros S, Glaser BM.  Low-dose colchicine inhibits astrocyte, fibroblast, and retinal pigment epithelial cell migration and proliferation . Arch Ophthalmol . 1986;;104:1223-1225.
8 +
Sunalp M, Wiedermann P, Sorgente N, Ryan SJ.  Effects of cytotoxic drugs on proliferative vitreoretinopathy in the rabbit cell injection model . Curr Eye Res . 1984;;3:619-623.
9 +
Meredith TA, Ficker L, Stevens R, et al.  Suppression of experimental tractional retinal detachment by low-dose radiation therapy . Arch Ophthalmol . 1988;;106:673-675.
10 +
Sternberg P, Meredith TA, Hartmann JN, Crocker IR.  Low-dose irradiation inhibits retinal pigment epithelial cell proliferation . In: Freeman HM, Tolentino FI, eds. Proliferative Vitreoretinopathy (PVR) . New York, NY: Springer-Verlag NY Inc; 1988;:140-144.
11 +
Belkin M, Avni I, Kessler E, Hercberg A, Spierer A, Treister G.  X-irradiation in the prevention of experimental post-traumatic vitreous proliferation . Curr Eye Res . 1982/1983;;2:753-756.
12 +
Binder S, Skorpik CH, Paroussis P, Kulnig W, Menapace R, Prokesch E.  High-energy electrons used to inhibit experimental intraocular proliferation and detachment . Ophthalmologica . 1987;;195:128-134.
13 +
Chakravarthy U, Gardiner TA, Maguire CJF, Archer DB.  Localised gamma irradiation and experimental intraocular proliferation . Trans Ophthalmol Soc UK . 1985;;104:792-799.
14 +
Dewey WC, Hopwood LE, Sapareto SA, Gerweck LE.  Cellular responses to combinations of hyperthermia and radiation . Radiology . 1977;;123:463-474.
15 +
Burgess SEP, Chang S, Svitra P, Driller J, Lizzi FL, Coleman DJ.  Effect of hyperthermia on experimental choroidal melanoma . Br J Ophthalmol . 1985;;69:854-860.
16 +
Finger PT, Packer S, Svitra PP, Paglione RW, Chess J, Albert DM.  Hyperthermic treatment of intraocular tumors . Arch Ophthalmol . 1984;;102:1477-1481.
17 +
Riedel KG, Svitra PP, Seddon JM, et al.  Proton beam irradiation and hyperthermia: effects on experimental choroidal melanoma . Arch Ophthalmol . 1985;;103:1862-1869.
18 +
Coleman DJ, Silverman RH, Iwamoto T, et al.  Histopathologic effects of ultrasonically induced hyperthermia in intraocular malignant melanoma . Ophthalmology . 1988;;95:970-981.
19 +
Lagendijk JJW.  A microwave heating technique for the hyperthermic treatment of tumors in the eye, especially retinoblastoma . Phys Med Biol . 1982;;27:1313-1324.
20 +
Hall EJ, Towle LR.  Biological effects of heat . Cancer Res . 1984;;44( (suppl) ):4708S-4713S.

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1 +
Radtke ND, Tano Y, Chandler D, Machemer R.  Simulation of massive periretinal proliferation by autotransplantation of retinal pigment epithelial cells in rabbits . Am J Ophthalmol . 1981;;91:76-87.
2 +
Machemer R, Horn DV, Aaberg TM.  Pigment epithelial proliferation in human retinal detachment with massive periretinal proliferation . Am J Ophthalmol . 1978;;85:181-191.
3 +
Glaser BM, Cardin A, Biscoe B.  Proliferative vitreoretinopathy: the mechanism of development of vitreoretinal traction . Ophthalmology . 1987;;94:327-332.
4 +
Chandler DB, Rozakis G, de Juan E, Machemer R.  The effect of triamcinolone acetonide on a refined experimental model of proliferative vitreoretinopathy . Am J Ophthalmol . 1985;;99:686-690.
5 +
Blumenkranz M, Hernandez E, Ophir A, Norton EWD. 5-Fluorouracil:  new applications in complicated retinal detachment for an established antimetabolite . Ophthalmology . 1984;;91:122-130.
6 +
Stern WH, Lewis GP, Erickson PA, et al.  Fluorouracil therapy for proliferative vitreoretinopathy after vitrectomy . Am J Ophthalmol . 1983;;96:33-42.
7 +
Lemor M, de Bustros S, Glaser BM.  Low-dose colchicine inhibits astrocyte, fibroblast, and retinal pigment epithelial cell migration and proliferation . Arch Ophthalmol . 1986;;104:1223-1225.
8 +
Sunalp M, Wiedermann P, Sorgente N, Ryan SJ.  Effects of cytotoxic drugs on proliferative vitreoretinopathy in the rabbit cell injection model . Curr Eye Res . 1984;;3:619-623.
9 +
Meredith TA, Ficker L, Stevens R, et al.  Suppression of experimental tractional retinal detachment by low-dose radiation therapy . Arch Ophthalmol . 1988;;106:673-675.
10 +
Sternberg P, Meredith TA, Hartmann JN, Crocker IR.  Low-dose irradiation inhibits retinal pigment epithelial cell proliferation . In: Freeman HM, Tolentino FI, eds. Proliferative Vitreoretinopathy (PVR) . New York, NY: Springer-Verlag NY Inc; 1988;:140-144.
11 +
Belkin M, Avni I, Kessler E, Hercberg A, Spierer A, Treister G.  X-irradiation in the prevention of experimental post-traumatic vitreous proliferation . Curr Eye Res . 1982/1983;;2:753-756.
12 +
Binder S, Skorpik CH, Paroussis P, Kulnig W, Menapace R, Prokesch E.  High-energy electrons used to inhibit experimental intraocular proliferation and detachment . Ophthalmologica . 1987;;195:128-134.
13 +
Chakravarthy U, Gardiner TA, Maguire CJF, Archer DB.  Localised gamma irradiation and experimental intraocular proliferation . Trans Ophthalmol Soc UK . 1985;;104:792-799.
14 +
Dewey WC, Hopwood LE, Sapareto SA, Gerweck LE.  Cellular responses to combinations of hyperthermia and radiation . Radiology . 1977;;123:463-474.
15 +
Burgess SEP, Chang S, Svitra P, Driller J, Lizzi FL, Coleman DJ.  Effect of hyperthermia on experimental choroidal melanoma . Br J Ophthalmol . 1985;;69:854-860.
16 +
Finger PT, Packer S, Svitra PP, Paglione RW, Chess J, Albert DM.  Hyperthermic treatment of intraocular tumors . Arch Ophthalmol . 1984;;102:1477-1481.
17 +
Riedel KG, Svitra PP, Seddon JM, et al.  Proton beam irradiation and hyperthermia: effects on experimental choroidal melanoma . Arch Ophthalmol . 1985;;103:1862-1869.
18 +
Coleman DJ, Silverman RH, Iwamoto T, et al.  Histopathologic effects of ultrasonically induced hyperthermia in intraocular malignant melanoma . Ophthalmology . 1988;;95:970-981.
19 +
Lagendijk JJW.  A microwave heating technique for the hyperthermic treatment of tumors in the eye, especially retinoblastoma . Phys Med Biol . 1982;;27:1313-1324.
20 +
Hall EJ, Towle LR.  Biological effects of heat . Cancer Res . 1984;;44( (suppl) ):4708S-4713S.

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