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THE CORNEA: Title and subTitle BreakIII. HYDRATION PROPERTIES OF EXCISED CORNEAL PIECES FREE

V. EVERETT KINSEY, Ph.D.; DAVID G. COGAN, M.D.
Arch Ophthalmol. 1942;28(2):272-284. doi:10.1001/archopht.1942.00880080090011
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The present investigations on corneal hydration (turgescence and/or deturgescence) were carried out with two objectives in mind: first, to obtain data on the physical-chemical properties of the cornea and, secondly, to clarify further1 the roles of the various structural components in maintaining corneal function. To accomplish these aims, it seemed best to determine the amount of turgescence of corneal pieces in different solutions and then to consider swelling of the whole cornea under a number of conditions. In the interests of simplicity the present paper will be confined to reporting the findings acquired from studying hydration of corneal pieces, while the results obtained from the use of whole corneas will be published in a following paper.

It has long been known that gross corneal sections possess an enormous swelling capacity. Chevreuil2 showed that 100 Gm. of cornea would take up 461 cc. of water and 370 cc. of

REFERENCES

Cogan, D. G., and Kinsey, V. E.:  The Cornea: I. Transfer of Water and Sodium Chloride by Osmosis and Diffusion Through the Excised Cornea , Arch. Ophth. 27:466 ( (March) ) 1942;
 II. Transfer of Water and Sodium Chloride by Hydrostatic Pressure Through the Excised Cornea , Arch. Ophth. 27:696 ( (April) ) 1942;.
Chevreuil:  De l'influence que l'eau exerce sur plusieurs substances azotées solides , Ann. clin. et physiol. 19:32-60, 1821;.
Donders, F. C.:  Imbibitionserscheinungen der Hornhaut und Sclerotica , Arch. f. Ophth. ( (pt. 1) ) 3:166-176, 1857;.
Schweigger-Seidel, F.:  Ueber die Grundsubstanz und die Zellen der Hornhaut des Auges , Ber. d. Gesellsch. d. Wissensch. zu Leipzig 21:305-359, 1869;.
Leber, T.:  Cirkulations- und Ernährungsverhältnisse des Auges , in Graefe, A., and Saemisch, E. T.: Handbuch der gesamten Augenheilkunde , ed. 2, Leipzig, Wilhelm Engelmann, 1903;, vol. 2, pt. 2, chap. 11, p. 363.
Bullot, G.:  On the Swelling of Organic Tissues—Researches on the Cornea , Am. J. Physiol. 12:297-303, 1904;.
Fischer, F. P.:  Ueber die Beschaffenheit der äusseren Bulbushüllen bei abnormen intraokularen Druck , Arch. f. Augenh. 103:1-75, 1930;.
The necessity of considering the pH when evaluating the effect of a salt on swelling in a particular strength acid or alkali has been repeatedly stressed in the literature, especially by Loeb. The influence of the pH on the results reported by Fischer may best be illustrated by an example. Fischer allowed a piece of cornea to swell in a solution composed of 5 cc. of 0.4 normal hydrochloric acid, 8 cc. of water and 12 cc. of 0.4 molar sodium chloride solution and in a solution containing the same dilution of acid with 12 cc. of 0.4 molar sodium acetate solution. The cornea swelled to 5.2 times its original thickness in the former solution and did not swell at all in the latter. Disregarding the slight increase in pH in both solutions due to the presence of the cornea itself, we found the pH to be 2.17 for the sodium chloride solution and 4.80 for the sodium acetate solution. Thus the difference in the degree of swelling may be entirely a pH effect and have nothing to do with the properties of the salt.
Heringa, G. C., and Weidinger, A.:  Structure and Importance of Connective Tissue: XI. Water Binding in the Intermediary Substance , Nederl. tijdschr. v. geneesk. 84:4907-4917, 1940;.
Leyns, W. F.; Heringa, C., and Weidinger, A.:  Water Binding Capacity of Cornea , Acta brev. Neerland. 10:25-26, 1940;.
Kunitz, M.:  Elasticity, Double Refraction and Swelling of Isoelectric Gelatin , J. Gen. Physiol. 13:565-606, 1930;.

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Cogan, D. G., and Kinsey, V. E.:  The Cornea: I. Transfer of Water and Sodium Chloride by Osmosis and Diffusion Through the Excised Cornea , Arch. Ophth. 27:466 ( (March) ) 1942;
 II. Transfer of Water and Sodium Chloride by Hydrostatic Pressure Through the Excised Cornea , Arch. Ophth. 27:696 ( (April) ) 1942;.
Chevreuil:  De l'influence que l'eau exerce sur plusieurs substances azotées solides , Ann. clin. et physiol. 19:32-60, 1821;.
Donders, F. C.:  Imbibitionserscheinungen der Hornhaut und Sclerotica , Arch. f. Ophth. ( (pt. 1) ) 3:166-176, 1857;.
Schweigger-Seidel, F.:  Ueber die Grundsubstanz und die Zellen der Hornhaut des Auges , Ber. d. Gesellsch. d. Wissensch. zu Leipzig 21:305-359, 1869;.
Leber, T.:  Cirkulations- und Ernährungsverhältnisse des Auges , in Graefe, A., and Saemisch, E. T.: Handbuch der gesamten Augenheilkunde , ed. 2, Leipzig, Wilhelm Engelmann, 1903;, vol. 2, pt. 2, chap. 11, p. 363.
Bullot, G.:  On the Swelling of Organic Tissues—Researches on the Cornea , Am. J. Physiol. 12:297-303, 1904;.
Fischer, F. P.:  Ueber die Beschaffenheit der äusseren Bulbushüllen bei abnormen intraokularen Druck , Arch. f. Augenh. 103:1-75, 1930;.
The necessity of considering the pH when evaluating the effect of a salt on swelling in a particular strength acid or alkali has been repeatedly stressed in the literature, especially by Loeb. The influence of the pH on the results reported by Fischer may best be illustrated by an example. Fischer allowed a piece of cornea to swell in a solution composed of 5 cc. of 0.4 normal hydrochloric acid, 8 cc. of water and 12 cc. of 0.4 molar sodium chloride solution and in a solution containing the same dilution of acid with 12 cc. of 0.4 molar sodium acetate solution. The cornea swelled to 5.2 times its original thickness in the former solution and did not swell at all in the latter. Disregarding the slight increase in pH in both solutions due to the presence of the cornea itself, we found the pH to be 2.17 for the sodium chloride solution and 4.80 for the sodium acetate solution. Thus the difference in the degree of swelling may be entirely a pH effect and have nothing to do with the properties of the salt.
Heringa, G. C., and Weidinger, A.:  Structure and Importance of Connective Tissue: XI. Water Binding in the Intermediary Substance , Nederl. tijdschr. v. geneesk. 84:4907-4917, 1940;.
Leyns, W. F.; Heringa, C., and Weidinger, A.:  Water Binding Capacity of Cornea , Acta brev. Neerland. 10:25-26, 1940;.
Kunitz, M.:  Elasticity, Double Refraction and Swelling of Isoelectric Gelatin , J. Gen. Physiol. 13:565-606, 1930;.

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