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TRANSPLANTATION OF OCULAR TISSUES TO THE CHORIOALLANTOIC MEMBRANE: Title and subTitle BreakObservations on Virus Inoculation FREE

H. P. KIRBER, M.D.; MARIA WIENER KIRBER, Ph.D.
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This study was supported by grants from the National Council to Combat Blindness, Inc.


Arch Ophthalmol. 1953;50(1):2-15. doi:10.1001/archopht.1953.00920030005002
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THE PRESERVATION of ocular tissues in a living state outside the human body is of interest because such tissues may support the growth of viruses affecting the eye and their mode of action and propagation can be studied. Some viruses have been successfully cultivated in animals or in the developing chick embryo (those of herpes simplex, vaccinia, lymphogranuloma venereum, epidemic keratoconjunctivitis) ; others, such as the viruses of trachoma and inclusion conjunctivitis, have up to now not been grown with certainty outside the human body.

This study deals with experiments in a large series of mammalian ocular tissues transplanted and kept viable on the chorioallantoic membrane of the developing chick embryo and the infection of such transplants with pathogenic ocular viruses.

MATERIALS AND METHODS  In 1912 Murphy1 successfully grafted mammalian tumor tissues onto the chick embryo. Since then a number of normal and tumor tissues from different species have been

REFERENCES

Murphy, J. B.:  Transplantability of Malignant Tumors to the Embryos of a Foreign Species , J. A. M. A. 59:874-875 ( (Sept. 14) ) 1912;.
Goodpasture, E. W.; Douglas, B., and Anderson, K.:  A Study of Human Skin Grafted upon the Chorio-Allantois of Chick Embryos , J. Exper. Med. 68:891-904 ( (Dec.) ) 1938;.
Blank, H.; Coriell, L. L., and Scott, T. F. M.:  Human Skin Grafted upon the Chorioallantois of the Chick Embryo for Virus Cultivation , Proc. Soc. Exper. Biol. & Med. 69:341-345 ( (Nov.) ) 1948;.
TenBroeck, C.:  Cultivation of the Hog Cholera Virus , J. Exper. Med. 74:427-432 ( (Nov.) ) 1941;.
Goodpasture, E. W., and Anderson, K.:  Infection of Human Skin, Grafted on the Chorio-Allantois of Chick Embryos, with the Virus of Herpes Zoster , Am. J. Path. 20:447-453 ( (May) ) 1944;.
Goodpasture, E. W., and Anderson, K.:  Virus Infection of Human Fetal Membranes Grafted on the Chorio-Allantois of Chick Embryos , Am. J. Path. 18:563-571 ( (July) ) 1942;.
Kirber, H. P.; Kirber, M. W., and Henle, W.:  Transplantation of Cornea to Chorio-Allantoic Membrane, with Observations on Virus Inoculation , Proc. Soc. Exper. Biol. & Med. 73:481-485 ( (March) ) 1950;.
Grignolo, A.:  Culture di tessuto corneale, conjunctivale, sclerale e uveale sulla corioallantoide di embrione di pollo , Boll. ocul. 29:617-637 ( (Oct.) ) 1950;.
Sezer, N.:  Cultivation of Conjunctival and Corneal Tissue on the Chorioallantoic Membrane , Arch. Ophth. 44:703-710 ( (Nov.) ) 1950;.
Sames, G. L., and Leathem, J. H.:  Influence of Desoxycorticosterone Acetate and Cortisone Acetate on Body Weight of Chick Embryos , Proc. Soc. Exper. Biol. & Med. 78:231-232 ( (Oct.) ) 1951;.
Jones, I. S., and Meyer, K.:  Inhibition of Vascularization of the Rabbit Cornea by Local Application of Cortisone , Proc. Soc. Exper. Biol. & Med. 74:102-104 ( (May) ) 1950;.
Sezer, F. N.:  Cultivation of Virus of Epidemic Keratoconjunctivitis on Chorioallantoic Membrane of Fertile Egg , A. M. A. Ophth. 49:293-302 ( (March) ) 1953;.

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Murphy, J. B.:  Transplantability of Malignant Tumors to the Embryos of a Foreign Species , J. A. M. A. 59:874-875 ( (Sept. 14) ) 1912;.
Goodpasture, E. W.; Douglas, B., and Anderson, K.:  A Study of Human Skin Grafted upon the Chorio-Allantois of Chick Embryos , J. Exper. Med. 68:891-904 ( (Dec.) ) 1938;.
Blank, H.; Coriell, L. L., and Scott, T. F. M.:  Human Skin Grafted upon the Chorioallantois of the Chick Embryo for Virus Cultivation , Proc. Soc. Exper. Biol. & Med. 69:341-345 ( (Nov.) ) 1948;.
TenBroeck, C.:  Cultivation of the Hog Cholera Virus , J. Exper. Med. 74:427-432 ( (Nov.) ) 1941;.
Goodpasture, E. W., and Anderson, K.:  Infection of Human Skin, Grafted on the Chorio-Allantois of Chick Embryos, with the Virus of Herpes Zoster , Am. J. Path. 20:447-453 ( (May) ) 1944;.
Goodpasture, E. W., and Anderson, K.:  Virus Infection of Human Fetal Membranes Grafted on the Chorio-Allantois of Chick Embryos , Am. J. Path. 18:563-571 ( (July) ) 1942;.
Kirber, H. P.; Kirber, M. W., and Henle, W.:  Transplantation of Cornea to Chorio-Allantoic Membrane, with Observations on Virus Inoculation , Proc. Soc. Exper. Biol. & Med. 73:481-485 ( (March) ) 1950;.
Grignolo, A.:  Culture di tessuto corneale, conjunctivale, sclerale e uveale sulla corioallantoide di embrione di pollo , Boll. ocul. 29:617-637 ( (Oct.) ) 1950;.
Sezer, N.:  Cultivation of Conjunctival and Corneal Tissue on the Chorioallantoic Membrane , Arch. Ophth. 44:703-710 ( (Nov.) ) 1950;.
Sames, G. L., and Leathem, J. H.:  Influence of Desoxycorticosterone Acetate and Cortisone Acetate on Body Weight of Chick Embryos , Proc. Soc. Exper. Biol. & Med. 78:231-232 ( (Oct.) ) 1951;.
Jones, I. S., and Meyer, K.:  Inhibition of Vascularization of the Rabbit Cornea by Local Application of Cortisone , Proc. Soc. Exper. Biol. & Med. 74:102-104 ( (May) ) 1950;.
Sezer, F. N.:  Cultivation of Virus of Epidemic Keratoconjunctivitis on Chorioallantoic Membrane of Fertile Egg , A. M. A. Ophth. 49:293-302 ( (March) ) 1953;.

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