0
July 1964, Vol 72, No. 1 >
ARTICLE | July 1964

The Fluorescent Antibody Technique in Diagnosis of Herpes Simplex Keratitis FREE

THOMAS H. PETTIT, MD; SAMUEL J. KIMURA, MD; HELEN PETERS
[+] Author Affiliations

Submitted for publication Jan 6, 1964.

Research supported in part by National Institutes of Health research grant No. NB-02542, and National Council to Combat Blindness grant-in-aid No. G-238.


Arch Ophthalmol. 1964;72(1):86-98. doi:10.1001/archopht.1964.00970020088020
Text Size: A A A
Published online
Article
References
Comments

The use of antibody labeled with fluorescein to localize specific antigens in various tissue preparations was originally described by Coons.1 It has since been used as a laboratory means of rapid diagnosis in a variety of microbiological systems and for the study of disease pathogenesis in a number of conditions in which an antigen-antibody system is involved.2

In 1959 Biegeleisen3 used this technique in differentiating clinically similar skin and mucus lesions due to herpes simplex virus and nonherpetic agents. He demonstrated an excellent correlation between the presence of positive fluoresecent staining and the ability to isolate the herpes virus from the lesions.3 The clinical usefulness of this test as a rapid diagnostic tool in herpetic keratitis has been suggested by the preliminary studies of Kaufman4 and Vozza and Balducci.5

The direct technique of Coons is based on the specificity of the antigen-antibody reaction which

REFERENCES

1 +
Kindly donated by the Pacific area office of the American National Red Cross, San Francisco.
2 +
We are indebted to Miss Birgitta Pehrsson and Dr. Phillips Thygeson for the electron microscopic studies of herpes-infected rabbit corneal epithelium, with which we made our comparisons.
3 +
Coons, A. H., and Kaplan, M. H.:  Localization of Antigen in Tissue Cells: II. Improvement in Method for Detection of Antigen by Means of Fluorescent Antibody , J Exp Med 91:1-13, 1950;.
4 +
Spink, W. W.:  The Young Investigator and His Fluorescent Antibody , JAMA 181:889-891, 1962;.
5 +
Biegeleisen, J. Z., Jr.; Scott, L. V.; and Lewis, V., Jr.:  Rapid Diagnosis of Herpes Simplex Virus Infections With Fluorescent Antibody , Science 129: 640-641, 1959;.
6 +
Kaufman, H. E.:  Diagnosis of Corneal Herpes Simplex Infection by Fluorescent Antibody Staining , Arch Ophthal 64:382-384, 1960;.
7 +
Vozza, R., and Balducci, D.:  Technique of Fluorescent Antibodies in Ophthalmology: Study of Herpes Simplex Vaccinia Keratoconjunctivitis and Human Trachomatous Infection , Amer J Ophthal 52:72-77, 1961;.
8 +
Lebrun, J.:  Cellular Localization of Herpes Simplex Virus With Fluorescent Antibody , Virology 2:496-510, 1956;.
9 +
Ross, R. W., and Orlans, E.:  Redistribution of Nucleic Acids and Appearance of Specific Antigen in HeLa Cells Infected With Herpes Virus , J Path Bact 76:393, 1958;.
10 +
Roizman, B.:  Virus Infection of Cells in Mitosis, Bearing on the Site of Virus Antigen Formation , Virology 13:387-401, 1961;.
11 +
Scott, T. F. McN., et al:  Growth Curve of Virus of Herpes Simplex in Rabbit Corneal Cells Grown in Tissue Culture With Parallel Observations on Development of Intranuclear Inclusion Body , J Immun 71:385-396, 1953;.
12 +
Kaufman, H. E., and Kaufman, E.:  Human Amnion Cell Tissue Culture: Study of Herpes Simplex and Adenovirus , AMA Arch Ophthal 59: 342-349, 1958;.
13 +
Smadel, J.: Viral and Rickettsial Infections in Man , 2nd ed, T. M. Rivers, ed., Philadelphia: J. B. Lippincott Company, 1952;, p 7.
14 +
Jawetz, E.; Melnick, J. L.; and Adelberg, E. A.: Review of Medical Microbiology , 5th ed, Los Altos, Calif: Lange Medical Publications, 1962;, p 323.
15 +
Riggs, J. L., et al:  Isothiocyanate Compounds as Fluorescein Labeling Agents for Immune Serum , Amer J Path 34:1081-1097, 1958;.
16 +
Marshall, J. C.; Eveland, W. C.; and Smith, C. W.:  Superiority of Fluorescein Isothiocyanate (Riggs) for Fluorescent-Antibody Technic With Modification of Its Application , Proc Soc Exp Biol Med 98:898-900, 1958;.
17 +
Morgan, C., et al:  Electron Microscopic Observations on Development of Herpes Simplex Virus , J Exp Med 110:643-656, 1959;.
18 +
Epstein, M. A.:  Observations on Mode of Release of Herpes Virus From Infected HeLa Cells , J Cell Biol 12:589-597, 1962;.

First Page Preview

First page PDF preview

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

1 +
Kindly donated by the Pacific area office of the American National Red Cross, San Francisco.
2 +
We are indebted to Miss Birgitta Pehrsson and Dr. Phillips Thygeson for the electron microscopic studies of herpes-infected rabbit corneal epithelium, with which we made our comparisons.
3 +
Coons, A. H., and Kaplan, M. H.:  Localization of Antigen in Tissue Cells: II. Improvement in Method for Detection of Antigen by Means of Fluorescent Antibody , J Exp Med 91:1-13, 1950;.
4 +
Spink, W. W.:  The Young Investigator and His Fluorescent Antibody , JAMA 181:889-891, 1962;.
5 +
Biegeleisen, J. Z., Jr.; Scott, L. V.; and Lewis, V., Jr.:  Rapid Diagnosis of Herpes Simplex Virus Infections With Fluorescent Antibody , Science 129: 640-641, 1959;.
6 +
Kaufman, H. E.:  Diagnosis of Corneal Herpes Simplex Infection by Fluorescent Antibody Staining , Arch Ophthal 64:382-384, 1960;.
7 +
Vozza, R., and Balducci, D.:  Technique of Fluorescent Antibodies in Ophthalmology: Study of Herpes Simplex Vaccinia Keratoconjunctivitis and Human Trachomatous Infection , Amer J Ophthal 52:72-77, 1961;.
8 +
Lebrun, J.:  Cellular Localization of Herpes Simplex Virus With Fluorescent Antibody , Virology 2:496-510, 1956;.
9 +
Ross, R. W., and Orlans, E.:  Redistribution of Nucleic Acids and Appearance of Specific Antigen in HeLa Cells Infected With Herpes Virus , J Path Bact 76:393, 1958;.
10 +
Roizman, B.:  Virus Infection of Cells in Mitosis, Bearing on the Site of Virus Antigen Formation , Virology 13:387-401, 1961;.
11 +
Scott, T. F. McN., et al:  Growth Curve of Virus of Herpes Simplex in Rabbit Corneal Cells Grown in Tissue Culture With Parallel Observations on Development of Intranuclear Inclusion Body , J Immun 71:385-396, 1953;.
12 +
Kaufman, H. E., and Kaufman, E.:  Human Amnion Cell Tissue Culture: Study of Herpes Simplex and Adenovirus , AMA Arch Ophthal 59: 342-349, 1958;.
13 +
Smadel, J.: Viral and Rickettsial Infections in Man , 2nd ed, T. M. Rivers, ed., Philadelphia: J. B. Lippincott Company, 1952;, p 7.
14 +
Jawetz, E.; Melnick, J. L.; and Adelberg, E. A.: Review of Medical Microbiology , 5th ed, Los Altos, Calif: Lange Medical Publications, 1962;, p 323.
15 +
Riggs, J. L., et al:  Isothiocyanate Compounds as Fluorescein Labeling Agents for Immune Serum , Amer J Path 34:1081-1097, 1958;.
16 +
Marshall, J. C.; Eveland, W. C.; and Smith, C. W.:  Superiority of Fluorescein Isothiocyanate (Riggs) for Fluorescent-Antibody Technic With Modification of Its Application , Proc Soc Exp Biol Med 98:898-900, 1958;.
17 +
Morgan, C., et al:  Electron Microscopic Observations on Development of Herpes Simplex Virus , J Exp Med 110:643-656, 1959;.
18 +
Epstein, M. A.:  Observations on Mode of Release of Herpes Virus From Infected HeLa Cells , J Cell Biol 12:589-597, 1962;.

Correspondence

Submit a Letter
CME Course for:


You need to register in order to view this quiz.


To understand the clinical management of acute heart failure syndromes.
Accreditation Information The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians.
The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity.
Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
Note: You must get at least of the answers correct to pass this quiz.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.
To view and print your certificate and access a summary of your CME courses go to My CME.
NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s “Cited By” API will populate this tab (http://www.crossref.org/citedby.html).
Compression-Only CPR: Pushing the Science Forward
Cone
AAM 2010;304:1493-1495.
All you need to read in the other general journals
BMJ 2010;341:c5893-c1494.
Submit a Comment

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

© 2012 American Medical Association. All Rights Reserved.
Powered bySilverchair Information Systems