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ARTICLE |

Cell Origin of Collagenase in Normal and Wounded Corneas FREE

Stuart I. Brown, MD; Carol A. Weller, PhD
[+] Author Affiliations

Submitted for publication May 8, 1969.

Reprint requests to the Department of Ophthalmology, Cornea Research Laboratory, New York Hospital-Cornell University Medical Center, 525 E 68th St, New York 10021 (Dr. Brown).


Arch Ophthalmol. 1970;83(1):74-77. doi:10.1001/archopht.1970.00990030076014
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Collagenase was not produced in detectable quantities by the normal rabbit cornea. However, the corneas with full thickness wounds did produce enzyme. The epithelium contributed the great majority of the enzyme, and the production appeared to be triggered by an interaction with the stromal wound.

REFERENCES

Gross, J., and Lapiere, C.M.:  Collagenolytic Activity in Amphibian Tissues: A Tissue Culture Assay , Proc Nat Acad Sci 48:1014 ( (April) ) 1962;.
Grillo, H.C., and Gross, J.:  Collagenolytic Activity During Mammalian Wound Repair , Develop Biol 15:300 ( (April) ) 1967;.
Eisen, A.Z.; Jeffrey, J.J.; and Gross, J.:  Human Skin Collagenase: Isolation and Mechanism of Attack on the Collagen Molecule , Biochim Biophys Acta 151:637-645, 1968;.
Lazarus, G.S., et al:;  Human Granulocyte Collagenase , Science 159:1483-1485 ( (Dec) ) 1968;.
Slansky, H.H.; Freeman, M.I.; and Itoi, M.:  Collagenolytic Activity in Bovine Corneal Epithelium , Arch Ophthal 80:496-498 ( (Oct) ) 1968;.
Brown, S.I.; Weller, C.A.; and Wassermann, H.E.:  Collagenolytic Activity of Alkali-Burned Corneas , Arch Ophthal 81:370-373 ( (March) ) 1969;.
Gross, J.:  Studies on the Formation of Collagen I. Properties and Fractionation of Neutral Salt Extracts of Normal Guinea Pig Connective Tissue , J Exp Med 107:247-263, 1958;.

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Gross, J., and Lapiere, C.M.:  Collagenolytic Activity in Amphibian Tissues: A Tissue Culture Assay , Proc Nat Acad Sci 48:1014 ( (April) ) 1962;.
Grillo, H.C., and Gross, J.:  Collagenolytic Activity During Mammalian Wound Repair , Develop Biol 15:300 ( (April) ) 1967;.
Eisen, A.Z.; Jeffrey, J.J.; and Gross, J.:  Human Skin Collagenase: Isolation and Mechanism of Attack on the Collagen Molecule , Biochim Biophys Acta 151:637-645, 1968;.
Lazarus, G.S., et al:;  Human Granulocyte Collagenase , Science 159:1483-1485 ( (Dec) ) 1968;.
Slansky, H.H.; Freeman, M.I.; and Itoi, M.:  Collagenolytic Activity in Bovine Corneal Epithelium , Arch Ophthal 80:496-498 ( (Oct) ) 1968;.
Brown, S.I.; Weller, C.A.; and Wassermann, H.E.:  Collagenolytic Activity of Alkali-Burned Corneas , Arch Ophthal 81:370-373 ( (March) ) 1969;.
Gross, J.:  Studies on the Formation of Collagen I. Properties and Fractionation of Neutral Salt Extracts of Normal Guinea Pig Connective Tissue , J Exp Med 107:247-263, 1958;.

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