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Peroxidase Diffusion Processes in the Optic Nerve FREE

Gholam A. Peyman, MD; David Apple, MD
[+] Author Affiliations

Submitted for publication April 21, 1972.

Reprint requests to the Department of Ophthalmology, 1855 W Taylor St, Chicago 60612 (Dr. Peyman).


Arch Ophthalmol. 1972;88(6):650-654. doi:10.1001/archopht.1972.01000030652017
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Diffusion processes into and out of optic nerve capillaries and between vitreous and optic nerves were studied with peroxidase in squirrel monkeys. Following intravenous injection of peroxidase, no passage of tracer out of optic nerve capillaries occurred. Diffusion was obstructed by zonulae occludentes of capillary endothelium. After intravitreal injection, tracer diffused freely through the internal limiting membrane into intercellular spaces of the optic nerve parenchyma. Zonula occludentes prevented flow from the perivascular spaces of the optic nerve into the capillary lumen. A blood-nerve diffusion barrier thus exists in the optic nerve.

REFERENCES

Fuchs E:  Die pheriphere atrophie des sehnerven . Graefe Arch Ophthalmol 31:177-200, 1885;.
Greeff R:  Das Wesen der Fuchs'schen Atrophie im Sehnerv , in IX Congres international d'Ophtalmologie d'Utrecht . Berlin, Karger, 1899;, pp 237-341.
Jacoby E:  Ueber die Neurolgia des Sehnerven . Klin Monatsbl Augenheilkd 43:129-137, 1905;.
Kuhnt H:  Zur Kentniss des Sehnerven und der Netzhaut . Graefe Arch Ophthalmol 25:179-288, 1889;.
Elschnig A: Der normale Sehnerveneneintritt des menschlichen Auges . Wein, Gerold, 1900;.
Yamamoto T:  Electronmicroscopic observation of human optic nerves . Acta Soc Ophthalmol Jap 69:1527-1580, 1965;.
Cohen AI:  Ultrastructural aspects of the human optic nerve . Invest Ophthalmol 6:294-308, 1967;.
Anderson DR, Hoyt WF:  Ultrastructure of intraorbital portion of human and monkey optic nerve . Arch Ophthalmol 82:507-530, 1967;.
Anderson DR:  Ultrastructure of meningeal sheaths: Normal human and monkey optic nerve . Arch Ophthalmol 82:659-674, 1969;.
Anderson DR:  Ultrastructure of human and monkey lamina cribosa and optic nerve head . Arch Ophthalmol 82:800-814, 1969;.
Hayreh SS:  Posterior drainage of intraocular fluid from the vitreous . Exp Eye Res 5:123-144, 1966;.
Gartner J:  Electronenmikroskopische Beobachtungen an der Papille des Rattenauges und beim Papillenodem des Menschen . Ophthalmologica 153:367-384, 1967;.
Graham RC Jr, Karnovsky MJ:  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique . J Histochem Cytochem 14:291-302, 1966;.
Hogan MJ, Feeny L:  The ultrastructure of the retinal vessels: II. The small vessels . J Ultrastruct Res 9:29-46, 1963;.
Bernstein MH, Hollenberg MJ:  Movement of electron-opaque markers through the capillaries of the retina , in Rohen JW (ed.): The Structure of the Eye: II. Symposium . Stuttgart, Germany, Schattauer-Verlag, 1965;, p 129-138.
Peyman GA, Bok D:  Peroxidase diffusion in the normal and laser-coagulated primate retina . Invest Ophthalmol 11:35-45, 1972;.

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Fuchs E:  Die pheriphere atrophie des sehnerven . Graefe Arch Ophthalmol 31:177-200, 1885;.
Greeff R:  Das Wesen der Fuchs'schen Atrophie im Sehnerv , in IX Congres international d'Ophtalmologie d'Utrecht . Berlin, Karger, 1899;, pp 237-341.
Jacoby E:  Ueber die Neurolgia des Sehnerven . Klin Monatsbl Augenheilkd 43:129-137, 1905;.
Kuhnt H:  Zur Kentniss des Sehnerven und der Netzhaut . Graefe Arch Ophthalmol 25:179-288, 1889;.
Elschnig A: Der normale Sehnerveneneintritt des menschlichen Auges . Wein, Gerold, 1900;.
Yamamoto T:  Electronmicroscopic observation of human optic nerves . Acta Soc Ophthalmol Jap 69:1527-1580, 1965;.
Cohen AI:  Ultrastructural aspects of the human optic nerve . Invest Ophthalmol 6:294-308, 1967;.
Anderson DR, Hoyt WF:  Ultrastructure of intraorbital portion of human and monkey optic nerve . Arch Ophthalmol 82:507-530, 1967;.
Anderson DR:  Ultrastructure of meningeal sheaths: Normal human and monkey optic nerve . Arch Ophthalmol 82:659-674, 1969;.
Anderson DR:  Ultrastructure of human and monkey lamina cribosa and optic nerve head . Arch Ophthalmol 82:800-814, 1969;.
Hayreh SS:  Posterior drainage of intraocular fluid from the vitreous . Exp Eye Res 5:123-144, 1966;.
Gartner J:  Electronenmikroskopische Beobachtungen an der Papille des Rattenauges und beim Papillenodem des Menschen . Ophthalmologica 153:367-384, 1967;.
Graham RC Jr, Karnovsky MJ:  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique . J Histochem Cytochem 14:291-302, 1966;.
Hogan MJ, Feeny L:  The ultrastructure of the retinal vessels: II. The small vessels . J Ultrastruct Res 9:29-46, 1963;.
Bernstein MH, Hollenberg MJ:  Movement of electron-opaque markers through the capillaries of the retina , in Rohen JW (ed.): The Structure of the Eye: II. Symposium . Stuttgart, Germany, Schattauer-Verlag, 1965;, p 129-138.
Peyman GA, Bok D:  Peroxidase diffusion in the normal and laser-coagulated primate retina . Invest Ophthalmol 11:35-45, 1972;.

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